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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Description of key information

The 72-h ErC50 value is >100 mg/L and the 72-h NOErC is <100 mg/L in Pseudokirchneriella subcapitata

Key value for chemical safety assessment

EC50/LC50 for freshwater algae:
100 mg/L
EC10, LC10 or NOEC for freshwater algae:
100 mg/L

Additional information

The effect of EEA-NH4 on the growth of the freshwater microalga Pseudokirchneriella subcapitata was determined in a GLP-compliant study according to OECD guideline 201 (Kurume Laboratory, 2006d).

In this static limit test algae (initial cell concentration of 10E+4 cells/mL) were exposed for 72 hours to 100 mg/L EEA-NH4 or a culture medium control (6 replicates per concentration). The concentrations of test substance were analysed with high performance liquid chromatography (HPLC) at the start and end of exposure and determined at 96.4 and 98.6 percent of nominal concentration respectively.

Slight, yet significant differences (p<0.05) were observed on both areas under the growth curves and growth rate (7.24 and 1.78% respectively). Based on these findings both the ErC50 and EbC50 were determined at >100 mg/L, and the NOErC and NOEbC were determined at <100 mg/L.