Blue MGi 1037-Na (notification presscake)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2007-06-12 to 2007-06-21

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

The test medium was prepared by dissolving 55.1 mg of test item completely in 500 mL of test water using ultrasonic treatment for 10 minutes and intense stining for 10 minutes at room temperature. The test medium was prepared just before introduction of the daphnids.

Test organisms (species):

Daphnia magna

Details on test organisms:

At test start, the test organisms were ≤ 24 hours old and not first brood progeny.

Test type:

static

Water media type:

other: Reconstituted test water

Limit test:

yes

Total exposure duration:

48 h

Hardness:

not specified

Test temperature:

20°C

pH:

7.8 - 7.9

Dissolved oxygen:

≥ 8.7 mg/L

Salinity:

not specified

Conductivity:

not specified

Nominal and measured concentrations:

Nominal comcentration was 110 mg/L
Mean measured concentration 93 mg/L

Details on test conditions:

A 16-hour light to 8-hour dark cycle was used with a 30 minute transistion period. Light intensity during the light period was approximately 490 to 650 Lux. Four replicates of five daphnids each were used.

Reference substance (positive control):

not specified

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 110 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

110 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

yes

Conclusions:

No immobilized test organisms were observed during the test period of 48 hours in the treatments. Thus, the 48-hour NOEC and the 48-hour EC50 of Blue MGi 1037-Na to Daphnia magna were determined to be at ≥ 100 mg/L.

Executive summary:

The purpose of the 48-hour toxicity test was to evaluate the effect of the test item Blue MGi 1037-Na on the mobility of Daphnia magna. A limit test was performed in accordance with the test guidelines to demonstrate that the test item has no toxic effect on the test organisms up to and incruding a nominal concentration of 100 mg/L. The 48-hour NOEC of Blue MGi 1037-Na to Daphnia magna was determined to be >100 mg/L. The 48-hour EC50 were determined to be greater than 100 mg/L.

Description of key information

In a 48-hour acute toxicity test on Daphnia magna, the obtained results showed that the test item had no toxic effects on the mobility of Daphnia up to the concentration of 100 mg/L. The 48-h EC50 was determined to be higher than 100 mg/L and the 48-h NOEC was determined to be 100 mg/L.

Additional information

Key:

The purpose of the 48-hour toxicity test was to evaluate the effect of the test item Blue MGi 1037-Na on the mobility of Daphnia magna. A limit test was performed in accordance with the test guidelines to demonstrate that the test item has no toxic effect on the test organisms up to and incruding a nominal concentration of 100 mg/L. The 48-hour NOEC of Blue MGi 1037-Na to Daphnia magna was determined to be >100 mg/L. The 48-hour EC50 were determined to be greater than 100 mg/L.