Blue MGi 1037-Na (notification presscake)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2007-06-06 to 2007-07-12

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Analytical monitoring:

yes

Test organisms (species):

other: zebra fish (Brachydanio rerio)

Details on test organisms:

The mean body length of the fish was 2.1± 0.1 cm, and the mean body weight was 0.10 ± 0.02 g, means of 10 individual fish after acclimation. Seven days acclimation period.

Test type:

semi-static

Water media type:

other: Dechlorinated drinking water

Limit test:

yes

Total exposure duration:

96 h

Hardness:

between 10 and 250 mg CaCO3 per liter, not further specified

Test temperature:

23 ± 1°C

pH:

7.62 to 8.16

Dissolved oxygen:

> 80%

Salinity:

not specified

Conductivity:

not specified

Nominal and measured concentrations:

nominal: 100 mg/L
mean measured: 110 mg/L

Details on test conditions:

The light-dark cycle was 12h:12h and the limit test was carried out with 20 fishes (2 groups á 10 fishes).

Reference substance (positive control):

yes

Remarks:

K2Cr2O7 (A.R.)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

The 24h-LC50 of the potassium dichromate (A.R.) was determined to be 222 mg/L, which fulfilled the criteria for a valid test that the 24h-LC50 of the potassium dichromate should range 200 mg/L to 400 mg/L.

Validity criteria fulfilled:

yes

Conclusions:

Blue MGi 1037-Na had no acute toxic effects on zebra fish under the present conditions of the test. The 96h-NOEC was determined to be 100 mg/L (mean measured concentration 110 mg/L). The 96h-LOEC and 96h-LC50 were greater than 100 mg/L (mean measured concentration 110 mg/L). Based on these results and the acute toxicity grading criteria in the Guidelines for the Hazard Evaluation of New Chemical Substances (HJ/T154-2004), the hazardous level of the test substance is slight.

Executive summary:

The acute toxicity effect of the test substance Blue MGi 1037-Na to zebra fish (Brachydanio rerio) was determined in a 96-hour semi-static test with a daily test medium renewal. A limit test was performed in accordance with the result of the preliminary test to demonstrate that the test

substance at a loading rate of 100 mg/L has no acute toxicity effect on the test organisms. Thus, only the treatments at a loading rate of 100 mg/L and the controls were tested. The analytically measured test substance concentrations in the analyzed test medium samples were 110 mg/L at both the beginning and the end of the test. In the controls and the treatments at the loading rate of 100 mg/L (mean measured concentration 110 mg/L) no fish death was determined during the limit test period of 96 hours. Therefore, the 96h-NOEC was determined to be 100 mg/L (mean measured concentration 110 mg/L). The 96h-LOEC and 96h-LC50 were greater than 100 mg/L (mean measured concentration 110 mg/L). Based on these results and the acute toxicity grading criteria in the Guidelines for the Hazard Evaluation of New Chemical Substances (HJ/T154-2004), the hazardous level of the test substance is slight.

Description of key information

In a 96-hour acute toxicity test on Zebrafish (Danio rerio) the test item had no toxic effect on fish up to the limit concentration of 100 mg/L. Accordingly, the 96-h LC50 value was determined to be > 100 mg/L. The 96-h NOEC was determined to be 100 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

Key:

The acute toxicity effect of the test substance Blue MGi 1037-Na to zebra fish (Brachydanio rerio) was determined in a 96-hour semi-static test with a daily test medium renewal. A limit test was performed in accordance with the result of the preliminary test to demonstrate that the test substance at a loading rate of 100 mg/L has no acute toxicity effect on the test organisms. Thus, only the treatments at a loading rate of 100 mg/L and the controls were tested. The analytically measured test substance concentrations in the analyzed test medium samples were 110 mg/L at both the beginning and the end of the test. In the controls and the treatments at the loading rate of 100 mg/L (mean measured concentration 110 mg/L) no fish death was determined during the limit test period of 96 hours. Therefore, the 96h-NOEC was determined to be 100 mg/L (mean measured concentration 110 mg/L). The 96h-LOEC and 96h-LC50 were greater than 100 mg/L (mean measured concentration 110 mg/L). Based on these results and the acute toxicity grading criteria in the Guidelines for the Hazard Evaluation of New Chemical Substances (HJ/T154-2004), the hazardous level of the test substance is slight.