4'-aminoazobenzene-4-sulphonic acid

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

calculation (if not (Q)SAR)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

accepted calculation method

Justification for type of information:

Data is from computational model developed by USEPA

Qualifier:

according to guideline

Guideline:

other: Modeling database

Principles of method if other than guideline:

Long term toxicity of test chemical was performed on the aquatic invertebrate daphnia according to the EPISUITE, ECOSAR.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

- Common name: Water flea

Test type:

flow-through

Water media type:

freshwater

Total exposure duration:

21 d

Reference substance (positive control):

not specified

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

1.274 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

immobilisation

Remarks on result:

other: ECOSAR class

Validity criteria fulfilled:

no

Conclusions:

On the basis of no effects observed in a flow-through freshwater system, the NOEC value for the substance is estimated to be 1.274 mg/l for fish for 21 days of exposure duration.

Executive summary:

Based on the prediction done using ECOSAR class, the long term toxicity on aquatic invertebrate daphnia was predicted for test substance. On the basis of no effects observed in a flow-through freshwater system, the NOEC value for the substance is estimated to be 1.274 mg/l for aquatic invertebrate for 21 days of exposure duration. Based on this value, it can be concluded that the test chemical can be considered as non-toxic to daphnia at environmentally relevant concentrations and can be considered as not-classified as per the CLP classification criteria. 

Description of key information

Based on the prediction done using ECOSAR class, the long term toxicity on aquatic invertebrate daphnia was predicted for test substance. On the basis of no effects observed in a flow-through freshwater system, the NOEC value for the substance is estimated to be 1.274 mg/l for aquatic invertebrate for 21 days of exposure duration. Based on this value, it can be concluded that the test chemical can be considered as non-toxic to daphnia at environmentally relevant concentrations and can be considered as not-classified as per the CLP classification criteria. 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1.274 mg/L

Additional information

Based on the various long term studies available for the test chemical and structually and functionally similar read across chemical, studies were reviewed to determine the toxic nature of test chemical on the mobility and reproduction of aquatic invertebrates. The studies are as mentioned below:

 

Based on the prediction done using ECOSAR class, the long term toxicity on aquatic invertebrate daphnia was predicted for test substance. On the basis of no effects observed in a flow-through freshwater system, the NOEC value for the substance is estimated to be 1.274 mg/l for aquatic invertebrate for 21 days of exposure duration. Based on this value, it can be concluded that the test chemical can be considered as non-toxic to daphnia at environmentally relevant concentrations and can be considered as not-classified as per the CLP classification criteria. 

 

Similar acute Immobilization Test of test chemical was studied on Daphnia magna by providing the exposure period of 21 days. Test was performed according to the OECD Guideline 211 (Daphnia magna Reproduction Test). Test performed in the semi-static system. Control, 100, 32, 10 mg/L nominal concentration was added in the test vessel filled with 80 ml of test solution. Test conducted in the ten replicates, 10 daphnia magna per concentration (1 per vessel was added). Based on the effect of chemical on the normal activity of daphnia magna after 21 days, the EC50 was determine to ≥ 100 mg/l and NOEC was observed at 100 mg/l. Thus based on the EC50, it can be concluded that the test chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria. 

 

In the third study which was conducted to determine the long term toxicity of test chemical on the mortality rate of daphnia magna. Test conducted in the open system under the semi-static method. 4 replicates were used in which 10 organisms were used in each replicates. Control of DMSO: HCO-40 was 9:1 (100 mg/kg). Test conducted on the nominal concentration for 7 days, 14 days and 21days. After the exposure of chemical lethal concentration was measured. Based on the mortality rate of daphnia magna due to the long period exposure with chemical, the LC50 value was observed at 13 mg/l, 10 mg/l and 9.7 mg/l for 7 days, 14 days and 21 days of exposure. The EC50 was 4.4 and 9.1 after 14 and 21 days of exposure. Based on the LC50, it can be concluded that the chemical was nontoxic and not classified as per the CLP classification criteria.

 

Thus based on the above effects and studies available on the long term effect on aquatic invertebrate, test chemical consider to be nontoxic to the aquatic invertebrates and hence not classified as per the CLP classification criteria.