4'-aminoazobenzene-4-sulphonic acid

Long-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to fish, other

Type of information:

calculation (if not (Q)SAR)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

accepted calculation method

Justification for type of information:

Data is from computational model developed by USEPA

Qualifier:

according to guideline

Guideline:

other: Modelling database

Principles of method if other than guideline:

Long term toxicity to fish was carried out according to the EPISUITE, ECOSAR.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

other: Fish

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

28 d

Reference substance (positive control):

no

Key result

Duration:

28 d

Dose descriptor:

NOEC

Effect conc.:

53.554 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: ECOSAR class

Validity criteria fulfilled:

no

Conclusions:

On the basis of no effects observed in a semi-static freshwater system, the NOEC value for the substance is estimated to be 53.554 mg/l for fish for 28 days of exposure duration

Executive summary:

Based on the prediction done using ECOSAR class, the long term toxicity on fish was predicted for test substance. On the basis of no effects observed in a semi- static freshwater system, the NOEC value for the substance is estimated to be 53.554 mg/l for fish for 28 days of exposure duration. Based on this value, it can be concluded that the test chemical can be considered as non-toxic to fish at environmentally relevant concentrations and can be considered not-classified as per the CLP classification criteria. 

Description of key information

Based on the prediction done using ECOSAR class, the long term toxicity on fish was predicted for test substance. On the basis of no effects observed in a semi- static freshwater system, the NOEC value for the substance is estimated to be 53.554 mg/l for fish for 28 days of exposure duration. Based on this value, it can be concluded that the test chemical can be considered as non-toxic to fish at environmentally relevant concentrations and can be considered not-classified as per the CLP classification criteria. 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

53.554 mg/L

Additional information

Various long term studies available for the test chemical and structually and functionally similar read across chemicals were reviewed to determine the toxic nature of test chemical on the mortality of fish. The studies are as mentioned below:

 

Based on the prediction done using ECOSAR class, the long term toxicity on fish was predicted for test substance. On the basis of no effects observed in a semi- static freshwater system, the NOEC value for the substance is estimated to be 53.554 mg/l for fish for 28 days of exposure duration. Based on this value, it can be concluded that the test chemical can be considered as non-toxic to fish at environmentally relevant concentrations and can be considered not-classified as per the CLP classification criteria. 

 

Above data was supported by the second study from authoritative database. Aim of this study was to evaluate the long term effect of test chemical on the fish. Test conducted in accordance with OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study). Before use chemical analytically monitored by HPLC. Test performed under the flow through system. 100, 50, 25.0, 12.5, 6.25 mg/L (geometric ratio 2.0), control nominal concentrations were used. Test liquid volume 3.6 L per concentration were added with 10 test organism per vessel. After the exposure of complete 21 days effect were measured. The lethal concentration (LC50) value of test chemical on freshwater fish in a 14 & 21 days study was determine to be 100 & > 100 mg/L and NOEC value was observed to be 25 mg/l. Based on this value, chemical consider to be nontoxic and can be consider to be not classified as per the CLP classification criteria.

 

Based on the above data, it can be concluded that the chemical was nontoxic to the mortality of fish and thus not classified as per the CLP classification criteria.