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EC number: 949-219-1
CAS number: -
Table 2: Mean
OD562Values and Viabilities for
the Negative Control Item, Positive Control Item and Test Item
MeanOD562of individual tissues
Mean OD562of duplicate tissues
Coefficient of Variation
Relative Mean Viability (%)
= Optical density
mean % viability of the negative control tissue is set at 100%
The skin corrosivity potential of reaction
mass of (4R)-4-(2-methoxypropan-2-yl)-1-methylcyclohexene and
cis-1-methoxy-4-(2-methoxypropan-2-yl)-1-methylcyclohexane was assessed
using an EpiDerm Reconstructed Human Epidermis (RHE) Model Kit in
accordance with OECD guidance 431 and GLP.
Duplicate tissues were treated
with the test item for exposure periods of 30 and 60 mins. At the end of
the exposure period the test item was rinsed from each tissue before
being loaded with MTT. After MTT loading each tissue was placed in 2 mL
isopropanol for MTT extraction. After extraction, each tissue was
pierced and the extraction solution aliquoted for absorbance
measurements. Absorbency at 562 nm of each well was measured using a
Anthos 2001 microplate reader.
viability of tissues exposed to the test substance after 3 and 60
minutes were 107.6 and 72.9 %, respectively. The quality criteria
required for acceptance of the resuts was met.
Under the conditions of this study
the test substance is not considered to be corrosive to the skin.
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