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EC number: 695-797-0
CAS number: 93601-86-6
Acceptability of the test:
- The positive control substance was
biodegraded by at least 60% (66%) within 14 days.
- The difference of duplicate values for
%-degradation of the test substance was always less than 20 (≤ 2%).
- The total CO2 release in the blank at the
end of the test did not exceed 40 mg/L (61.5 mg CO2 per 2 litres of
medium, corresponding to 30.8 mg CO2/L).
- The Inorganic Carbon content (IC) of the
test substance (suspension) in the mineral medium at the beginning of
the test was less than 5% of the Total Carbon content (TC). Since the
test medium was prepared in tap-water purified by reverse osmosis
(Milli-RO water (Millipore Corp., Bedford, Mass., USA, carbon levels <
500 ppb)), IC was less than 5% of TC (mainly coming from the test
substance, 12 mg TOC/L).
Since all criteria for acceptability of the
test were met, this study was considered to be valid.
The ready biodegradation of the substance
under the conditions of the carbon dioxide (CO2) evolution test
(modified Sturm test) was investigated according to OECD guideline 301 B
and GLP principles. A test concentration of 12 mg/L (as TOC) was tested
in duplicate during 28 days. The relative biodegradation values
calculated from the measurements performed during the test period
revealed no significant biodegradation (3% and 1% biodegradation of the
substance, for replicate A and B, respectively (based on ThCO2)). In the
toxicity control, the substance was found not to inhibit microbial
activity. The study is considered to be reliable without restrictions.
In conclusion, the substance was determined to be not readily
biodegradable under the conditions of the modified Sturm test.
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