Reaction mass of 3-phenoxybenzyl (1R,3R)-2,2-dimethyl-3-(2-methylprop-1-en-1-yl)cyclopropanecarboxylate and 3-phenoxybenzyl (1R,3S)-2,2-dimethyl-3-(2-methylprop-1-en-1-yl)cyclopropanecarboxylate (4:1)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2006

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The concentration of the test substance was not stable during the test. The test item likely absorbed to the glass wall or the test organisms.

Reason / purpose for cross-reference:

reference to other study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

The test concentration was measured at 0, 24, 48 an 96 hours.
The fish were checked for mortality after 2, 24, 48, 72 and 96 hours.

Vehicle:

yes

Details on test solutions:

0, 10, 17.8, 31.6, 56.2 and 100 µg/L, applied dissolved in ethanol and water.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Juvenile fish of the same age and size have been used. They had been kept for 14 days under stable conditions.During the holding period, they were fed up to 48 hours before the test.

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

No

Hardness:

Reconstituted test water: analytical grade salts were dissolved to obtain water hardness of 2.5 mmol l-1.

Test temperature:

21.7 - 23.2 °C

pH:

The pH ranged from 6-9 and varied not more than 0.4 units.

Dissolved oxygen:

The dissolved oxygen was ≥6.6 mg/L.

Salinity:

not applicable

Nominal and measured concentrations:

Nominal: 0, 10, 17.8, 31.6, 56.2 and 100 µg/L.
Measured: Levels could be detected only in 3 samples, presumably due to sorption of the test substance to the glass walls or to the test organisms.

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume:
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel:
- No. of vessels per concentration (replicates):
- No. of vessels per control (replicates):
- No. of vessels per vehicle control (replicates):
- Biomass loading rate:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16 hours
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

ca. 10 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

EC10

Effect conc.:

ca. 31.6 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 55.9 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: not available

Details on results:

While lowest mortality was observed at the lowest dose, and highest mortality at the highest dose, there was no consistent dose-response relationship. The intermediate doses showed lower mortality than the low dose, as is reported in the table below under "Any other information on results incl. tables".

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

Reference is made to Appendix C of the study report.

Sublethal observations / clinical signs:

 

Nominal test concentration [ug l-1]	No. of exposed fish	2h	24h	48h	72h	96h
0 (control)	7	0	0	0	0	0
0 (solvent control)	7	0	0	0	0	0
10	7	0	0	0	0	0
17.8	7	0	4	4	5	5
31.6	7	0	0	0	1*	2*
56.2	7	0	2	2	2*	3*
100	7	0	4	7	7	7

* All of the survivors showed signs of distress such as apathy and lateral swim and some were lying on one side

Validity criteria fulfilled:

yes

Conclusions:

Despite some deficiencies, the results are in line with other available data on d-Phenothrin.
EC0 = 10 µg/L
EC10 = 31.6 µg/L
LC50 = 55.9 µg/L

Executive summary:

EC0 = 10 µg/L

EC10 = 31.6 µg/L

LC50 = 55.9 µg/L

Description of key information

The acute aquatic toxicity to fish was found being 55.9 µg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

55.9 µg/L