2,4-dichlorophenol

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The SIDS dossier assigns a reliability of 1, valid without restrictions to this reference.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

secondary source

Title:

Unnamed

Year:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

not specified

GLP compliance:

yes

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

2,4-dichlorophenol

EC Number:

204-429-6

EC Name:

2,4-dichlorophenol

Cas Number:

120-83-2

Molecular formula:

C6H4Cl2O

IUPAC Name:

2,4-dichlorophenol

Details on test material:

The purity of the test substance is reported to be 99.6%.

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

No additional details presented.

Administration / exposure

Type of coverage:

semiocclusive

Vehicle:

unchanged (no vehicle)

Remarks:

Substance melted.

Duration of exposure:

Up to 9 days

Doses:

The dose levels were 200-300-1400 and 2000 mg/kg in males (5 animals/dose) and 200 and 2000 mg/kg in females (5 animals/dose).

No. of animals per sex per dose:

5

Control animals:

not specified

Details on study design:

The test substance was melted at 40 °C to obtain the liquid form to be tested. This was done in order to obtain a more pronounced toxic effect which can mimic accidents. The substance was applied under semi-occlusive patches during 24 h to the skin of Sprague-Dawley rats at a volume taking into consideration the density of the substance (d=1.38). The dose levels were 200-300-1400 and 2000 mg/kg in males (5 animals/dose) and 200 and 2000 mg/kg in females (5 animals/dose). The treated skin surface ranges between 2cm2 (200 mg/kg dose) and 30-35cm2 (2000 mg/kg dose).

Statistics:

No data

Results and discussion

Preliminary study:

No preliminary study reported.

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

780 mg/kg bw

Based on:

test mat.

Mortality:

There were 4/5 deaths at the highest dose level in both males and females. Death occurred within 6 days after application.

Clinical signs:

other: Slight decrease in motor activity was noted from 200 mg/kg and was no longer present 24 hours after application. Marked decrease in motor activity and respiratory impairment was observed from 300 mg/kg and 1400 mg/kg ; these signs were still present up to

Other findings:

At necropsy, presence of a liquid black in color in the urinary bladder of one male and one female at 2000 mg/kg.

Applicant's summary and conclusion

Interpretation of results:

harmful

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The LD50 was determined to be 780 mg/kg/bw

Executive summary:

The test substance was subjected to an acute dermal toxicity study according to OECD guideline 402. The study was performed to GLP. The LD50 was determined to be 780 mg/kg/bw. In addition, at all doses, marked to severe irritation resulting in skin necrosis was noted after removal of the patch, which was hardly reversible within 2 weeks.