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Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Japanese Industrial Standard (JIS K 0102-1986-71)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium hydrogen 4-amino-5-hydroxynaphthalene-2,7-disulphonate
EC Number:
226-736-4
EC Name:
Sodium hydrogen 4-amino-5-hydroxynaphthalene-2,7-disulphonate
Cas Number:
5460-09-3
Molecular formula:
C10H9NO7S2.Na
IUPAC Name:
sodium hydrogen 4-amino-5-hydroxynaphthalene-2,7-disulfonate

Test organisms

Test organisms (species):
Oryzias latipes
Details on test organisms:
- Supplier: Nakashima fish farm
- Conditions for fish keeping: At reception, fish showing abnormal signs were removed. The fish were reared according to flow -through system
for 3 - 5 weeks after external disinfection.
- Conditions for acclimatization: Fish were reared in an acclimatizatino tank according to flow-through system at temperature of 25°C +/- 2 for
about 28 d. During this period, abnormal fish were removed.

Study design

Water media type:
freshwater
Total exposure duration:
48 h

Test conditions

Test temperature:
25 °C +/- 2
Details on test conditions:
TEST CONDITIONS
- test tank: round glass vessel
- volume of test water: 4 L per aquaria
- number of fish: 10 per aquaria
- duration of exposure: 48 h
- origin of test water: underground water from the ground of Kurume Research Laboratories

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
880 mg/L
Conc. based on:
test mat.
Basis for effect:
mortality (fish)

Applicant's summary and conclusion