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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1-3 Sep 2008
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
Frequency at t=O h and t=48 h
Volume 2 ml from the approximate centre of the test vessels
Storage Samples were stored in a freezer until treatment.
Thereafter, samples were kept at room temperature until analysis.

On the day of analysis, the samples were defrosted at room temperature. The test samples were
diluted in a 1 :1 (v:v) ratio with 50/50 (v/v) acetonitrile/water containing 0.1 % (w/v) TMAH and
analysed. The samples at the high concentration level were 10-times further diluted with 50/50
(v/v) acetonitrile/water containing 0.05% (w/v) TMAH to obtain concentrations within the
calibration range.
The samples were treated on 08 September 2008 and analysed. With the analysis it was found
that the response of the system was inadequate. The analysis was repeated on 10 September
2008. The test samples were stored at room temperature. Stability of the samples under these
conditions was determined with the procedural recovery samples of this project.
Vehicle:
no
Details on test solutions:
The standard test procedures required generation of test solutions, which should contain
completely dissolved test substance concentrations or stable and homogeneous mixtures or
dispersions. The testing of concentrations that disturb the test system should be prevented (e.g.
film of the test substance on the water surface).
The batch of AHU377 C9 tested was a white powder with a purity of 99.82% and completely
soluble in test medium at the concentrations tested.
Preparation of test solutions started with a loading rate of 100 mg/I applying 30 minutes of
magnetic stirring followed by a 20-minute treatment period with ultrasonic waves and an
additional 1 Y. hours of stirring to accelerate the dissolving of the test substance in the test
medium. The lower test concentrations were prepared by subsequent dilutions of the 100 mg/I
concentration in test medium. The final test solutions were all clear and colourless.
Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna (Crustacea, Cladocera) (Straus,
1820), at least third generation, obtained by acyclical
parthenogenesis under specified breeding conditions.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Test temperature:
18-22°C
pH:
6.0-8.5
Dissolved oxygen:
ee3 mg/I
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Validity criteria fulfilled:
yes
Conclusions:
AHU377 C9 did not induce acute immobilisation of Daphnia magna at 100 mgl/l after 48 hours of exposure (NOEC). Hence, the 48h-EC,, was > 100 mgl/l.

Description of key information

AHU377 C9 did not induce acute immobilisation of Daphnia magna at 100 mgl/l after 48 hours of exposure (NOEC). Hence, the 48h-EC, was > 100 mgl/l.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information