Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: test according to OECD and GLP guidelines
Cross-reference
Reason / purpose:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1993
Report Date:
1993

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 99.9% confidence

Any other information on results incl. tables

no other information

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The maximum concentration causing no mortality within the test period after 96 h (LC0 was 100 mg/l. The minimum concentration causing 100 per cent mortality after 96 h (LC100) was >100 mg/l. The No Observed Effect Concentration after 96 h (NOEC) was 100 mg/l. According to binomial analysis the calculated LC(50) of C3-Phosphoniumbrornid after 96 h was > 100 mg/l with 99.9 % confidence.
Executive summary:

A static test procedure was used to assess the acute toxicity of the test substance C3-Phasphoniurnbromid to Rainbow Trout, oncorhynchus mykiss. The fish were exposed for 96 hours to aqueous solutions of the test substance at nominal test concentrations of 100.0, 66.7, 44.4, 29.6, and 19.8 mg/l. An additional group of fish was exposed to dilution water and served as a control. Observations regarding mortality and any visible abnormalities were made after 2, 24, 48, 72 and 96 hours. Temperature, pH, and oxygen concentration were measured in the test solutions at the beginning of the study and at 24-hour intervals thereafter throughout the study. There was no mortality in the control. The maximum concentration causing no mortality within the test period after 96 h (LC0 was 100 mg/l. The minimum concentration causing 100 per cent mortality after 96 h (LC100) was >l00 mg/l. The No Observed Effect Concentration after 96 h (NOEC) was 100 mg/l. According to binomial analysis the calculated LC(50) of C3-Phosphoniumbrornid after 96 h was > 100 mg/l with 99.9 % confidence.