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EC number: 249-949-4 | CAS number: 29911-27-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
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- Flash point
- Auto flammability
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- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
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- Dissociation constant
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- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
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- Nanomaterial aspect ratio / shape
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- Endpoint summary
- Stability
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- Environmental data
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
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- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Genetic toxicity
- Carcinogenicity
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- Specific investigations
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- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- October 10, 1997 - November 3, 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods
- Remarks:
- The study followed guidelines similar to U. S. EPA, EEC, OECD and MAFF guidelines and in accordance with the principles of GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 472 (Genetic Toxicology: Escherichia coli, Reverse Mutation Assay)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EPA OPPTS 870.5100 - Bacterial Reverse Mutation Test (August 1998)
- Deviations:
- no
- Principles of method if other than guideline:
- not applicable
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1-(1-methyl-2-propoxyethoxy)propan-2-ol
- EC Number:
- 249-949-4
- EC Name:
- 1-(1-methyl-2-propoxyethoxy)propan-2-ol
- Cas Number:
- 29911-27-1
- Molecular formula:
- C9H20O3
- IUPAC Name:
- 1-(1-methyl-2-propoxyethoxy)propan-2-ol
- Details on test material:
- Purity was 99.3%, contained 1.2 ppm peroxide
Constituent 1
Method
- Target gene:
- Histidine operon, rfa wall, uvr B gene
Species / strain
- Species / strain / cell type:
- other: S. typhimurium TA98, TA100, TA1535, TA1537 and E. coli WP2uvrA
- Details on mammalian cell type (if applicable):
- None
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S-9 (40.7 mg protein/ml) from male Sprague-Dawley rats that had been injected i.p. with 500 mg/kg Aroclor 1254
- Test concentrations with justification for top dose:
- 0, 100, 333, 1000, 3330 and 5000 micrograms/plate +/- S9 mix (initial study); 0, 313, 625, 1250, 2500 and 5000 micrograms/plate +/- S9 mix (confirmatory study). Analytical concentrations of stock solutions were 89-97% (initial study) and 95-99% (confirmatory study) of target.
- Vehicle / solvent:
- deionized water
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- 50 microliters deionized water
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene, 2-nitrofluorene, benzo(a)pyrene, sodium azide, ICR-191 or 4-nitroquinoline-N-oxide (depending on strain)
- Details on test system and experimental conditions:
- The tester strain and the S9 mix (where appropriate) were preincubated for 20 +/- 2 min at 37 +/- 2 degrees C prior to the addition of molten, selective overlay agar. The agar and preincubation reaction mixture were mixed and overlaid onto a minimal agar plate. Following incubation at 37 +/- 2 degrees C for 52 +/- 4 hr, revertant colonies were counted manually (vehicle control or test material) or by automated colony counter (positive controls). All doses of test material, vehicle and positive controls were tested in triplicate. The condition of the background lawn was evaluated for evidence of cytotoxicity or test material precipitation. Adequate tester strain integrity, initial density requirements and S9 activity were demonstrated.
- Evaluation criteria:
- For a test material to be considered positive, it had to produce at least a 3-fold (TA98, TA1535, TA 1537 and WP2uvrA) or 2-fold, dose-related and reproducible increase in the mean revertants per plate of at least one tester strain (compared to the vehicle control). A response that did not meet all 3 of the criteria (magnitude, dose-responsiveness, reproducibility) was not evaluated as positive.
- Statistics:
- For all replicate platings, the mean revertants per plate and the standard deviation were calculated.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- All criteria for a valid study were met. Both studies were negative.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results: negative
The results of the Salmonella - Escherichia coli/Marnmalian-Microsome Reverse Mutation Assay Preincubation Method with a Confirmatory Assay indicate that under the conditions of this study, test article Dipropylene glycol n-propyl ether (Dowanol DPnP), did not cause a positive increase in the number of revertants per plate of any of the tester strains either in the presence or absence of microsomal enzymes prepared from AroclorTM-induced rat liver (S9). - Executive summary:
Dipropylene glycol n-propyl ether (Dowanol DPnP) was evaluated in the Salmonella-Escherichia coli / mammalian-microsome bacterial mutagenicity assay using a pre-incubation modification of the standard method with a confirmatory assay. The test was conducted in the presence and absence of an externally supplied metabolic activation system (S-9) using Salmonella typhimurium tester strains TA98, TA100, TA1535, and TA1537 and Escherichia coli tester strain WP2uvrA.
The concentrations of the test material in the presence and absence of S9 mix ranged from 100 to 5000 pg per plate. The results were confirmed in an independent repeat assay.
The results indicate that Dowanol DPnP did not cause a positive increase in the number of revertants per plate of any of the tester strains in this bacterial mutagenicity test under the experimental conditions used.
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