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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

A 28-day CO2 evolution test (modified Sturm test) using microorganisms from domestic non-adapted sludge was performed under GLP conditions and according to OECD Guideline 301 B and EU Method C.4-C (Desmares-Koopmans, 2012).

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

In a 28-day CO2 evolution test (modified Sturm test) using microorganisms from domestic non-adapted sludge and performed under GLP, the biodegradation of the test substance was tested. As the test substance was not sufficiently soluble to allow preparation of an aqueous solution at a concentration of 1 g/l, the test solutions were continuously stirred during the test, to ensure optimal contact between the test substance and the test organisms.

The test substance showed to be not readily biodegradable; 1% biodegradation was observed after 28 days. No inhibition of microbial activity was observed (Desmares-Koopmans, 2012).