Registration Dossier

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

No study of toxicity to micro-organisms is available  for BHMT-H. A reliable study, performed with a Na/H salt of EDTMP, is available. The EC50 to WWTP micro-organisms was determined to be >100 mg/L as active acid. In view of the low level of inhibition seen at a loading of 100 mg/l, it is acceptable to interpret this value as an EC5. This result comes from a reliable study conducted according to an appropriate test protocol, and in compliance with GLP. 

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
100 mg/L

Additional information

5.4% inhibition was seen at 100 mg/L loading (in terms of active acid), thus the EC50 is >100 mg/l. No NOEC or EC5 was derived by the report authors, however in view of the low level of inhibition (approximately 5%) seen at a loading of 100 mg/l, it is considered acceptable to interpret this concentration as an approximation of the EC5 for the purpose of PNEC derivation.

The registration substance is part of a category family (super-category; see CSR for more details) which consists of several phosphonates that share a common chemistry incorporating alkyl backbones with one or more tertiary amine centres and multiple methylphosphonate groups present.


BHMT acid (CAS 34690-00-1) has three amine centres (five methylphosphonate groups), connected by hexyl chains. EDTMP acid (CAS 1429-50-1) has two aminobismethylphosphonate groups, connected by an ethyl chain.


As well as being structural analogues, both phosphonates have consistent chemical properties including high MW (685 and 436 respectively), very low log Kow(≤-4 for both substances) and are highly soluble in water. The substances generally posses similar physico-chemical properties and are not readily biodegradable.


The sodium counterion of EDTMP-xNa (22036-77-7) is not significant in respect of the properties under consideration and has been assessed in depth in the public literature.Additionally, the counterion is expected to dissociate when in contact with water, including atmospheric moisture.


Therefore, for the purpose of this assessment, read-across is considered valid. Further information on the super-category and the validity of read-across are presented in the CSR Chapter 1.