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EC number: 205-492-2 | CAS number: 141-63-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008-04-29 to 2008-05-05
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling: Samples were collected from one test chamber of each treatment and control group four days and two days prior to the start of the test after conditioning the diluter for approximately 45 and 96 hours respectively. Water samples were collected from alternating replicate test chambers in each treatment and control group at the beginning of the test and at 48 and 96 hours (+/-1 hour) to measure concentrations of the test substance. The samples were collected were collected from mid-depth into a 100 ml glass volumetric flask and analysed immediately.
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A primary stock was prepared by mixing a calculated amount of test substance into HPLC-grade dimethyl formamide (DMF) at a nominal concentration of 10 μg/ml. An aliquot of the primary stock solution was diluted with DMF to create a secondary stock solution at a concentration of 700 ng/l. Four additional stock solutions were prepared in DMF at nominal concentrations of 44, 88, 180 and 350 ng/ml. The stock solutions were mixed by inversion and appeared clear and colourless. Stock solutions were stored refrigerated in glass amber bottles or a 500 ml graduated cylinder, and aliquots of each stock were placed in the syringe every two days of the study.
The five test substance stock solutions were injected into the diluter mixing chambers at a rate of 20 μl/minute) where they were mixed with dilution water delivered at a rate of 200 mL/minute to achieve the desired test concentrations. The negative control received dilution water only. The solvent control was prepared by delivering HPLC-grade DMF to the mixing chamber for the solvent control. The DMF concentration in the solvent control and all L5 treatments was 0.1 ml/l. - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Test fish: Total Length 5.0 cm (Range 4.5 to 5.3 cm)/Wet Weight 0.94 g (Range 0.65 to 1.2 g).
- Pre-experiment holding period: 14 days
- Holding conditions: Similar to test conditions
- Feeding during holding: The fish were fed daily with a commercially-prepared diet supplied by Zeigler Brothers Inc., Gardners, PA., USA. The fish were not fed for two days prior to the test or during the test.
- Test loading: 0.065 g/L/day over 24 hours (flow through), 0.63 g/L (instantaneous). - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- Hardness 132 mg/L as CaCO3, Alkalinity 178 mg/L as CaCO3
- Test temperature:
- 12+/-1ºC
- pH:
- 8.0-8.2
- Dissolved oxygen:
- ≥7.2 mg/L
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentrations: Negative Control, Solvent Control, 4.4, 8.8, 18, 35 and 70 ng/L
Mean measured concentrations in treatments: 2.7, 7.5, 13.2, 35.1 and 75.1 ng/L.
Mean measured concentrations in treatments as a percentage of nominal: 61, 85, 73, 100 and 107%
The results are interpreted with reference to mean measured concentrations. - Details on test conditions:
- - Exposure vessel type: 25-L Teflon-lined stainless steel aquaria containing 15-L of test solution. The water depth was approximately 18 cm.
- Number of replicates, fish per replicate: Two replicates per treatment, 10 Fish per replicate
- Dilution water source: Fresh water obtained from a well approximately 40 m deep. The water was passed through a sand filter to remove particles approximately 25 μm in diameter and was aerated. Prior to use the water was again filtered (0.45 μm) and passed through a UV steriliser to remove microorganisms.
- Dilution water chemistry: Hardness 132 mg/L as CaCO3, Alkalinity 178 mg/L as CaCO3, Conductivity 323 mhos/cm
- Vehicle/solvent and concentrations: The solvent control and treatment groups contained 0.1 mL/L DMF
- Water chemistry in test: DO, pH and temperature measured in each test chamber daily - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 75 ng/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 75 ng/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- No mortalities or other signs of toxicity were observed in any treatment
- Reported statistics and error estimates:
- There was no significant mortality in any treatment; statistical analysis was therefore not required.
- Sublethal observations / clinical signs:
Table 1. Test results
Nominal concentration (ng/l) Mean measured concentration (ng/l) Percentage mortality after 24 hours Percentage mortality after 48 hours Percentage mortality after 72 hours Percentage mortality after 96 hours 0 (Control) - 0 0 0 0 0 (Vehicle control) - 0 0 0 0 4.4 2.7 0 0 0 0 8.8 7.5 0 0 0 0 18 13.2 0 0 0 0 35 35.1 0 0 0 0 70 75.1 0 0 0 0
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 96-hour LC50 value of >75 ng/L and NOEC of ≥75 ng/L have been determined for the effects of the substance on mortality of Oncorhynchus mykiss. The results have been obtained under flow-through conditions and are expressed relative to mean measured concentrations of the substance.
Reference
Description of key information
(96 hour) LC50: >75 ng/l mortality Oncorhynchus mykiss, reliability 1.
Key value for chemical safety assessment
Additional information
A 96 hour LC50 value of >75 ng/l and NOEC of ≥75 ng/l have been determined for the effects of the substance on mortality of Oncorhynchus mykiss. The results have been obtained under flow-through conditions and are expressed relative to mean measured concentrations of the substance.
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