Morpholinium sulphamate

Endpoint summary

Additional information

Hydrolysis

The performance of a test for hydrolysis as function of pH is scientifically unjustified. The study does not need to be conducted as morpholinium sulphamate completely dissociates in water forming morpholinium cation and the corresponding sulphamidic anion.

 

Biodegradation in water: screening test

The ready biodegradability test of Morpholinium Sulphamate was conducted according to OECD Guideline for Testing of Chemicals No. 301 D: “Ready Biodegradability: Closed Bottle Test”, adopted July 17, 1992, Commission Regulation (EC) No 440/2008, Annex Part C, C.4., Part VI: „Closed Bottle Test (Method C.4-E)“. Dated May 30, 2008 and EPA Guideline 712-C-98-076: OPPTS 835.3110, “Ready Biodegradability”, January 1998.

 

The test item was exposed to activated sludge from the aeration tank of a domestic waste water treatment plant for 28 days in closed bottles.

The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item Sodium benzoate was tested simultaneously under the same conditions as the test item.

Additionally a toxicity control has been conducted containing both, the test item and the reference item. The dissolved oxygen concentration was measured to calculate the percentage BOD. Furthermore because of the Nitrogen content of the test item, samples for nitrate and nitrite analysis were taken from all vessels (of test item, inoculum control and toxicity control group) and the oxidized Nitrogen (nitrate and nitrite) concentrations were measured after each oxygen measurement.

 

The reference item Sodium benzoate was sufficiently degraded to a mean of 76.1 % after 14 days, and to a mean of 88.0 % after 28 days of incubation, based on ThODNH4, thus confirming the suitability of the used activated sludge inoculum.

In the toxicity control a mean of 39.1 % biodegradation was noted within 14 days and 41.4 % biodegradation after 28 days of incubation.

Thus, the test item can be assumed to not inhibit the activated sludge microorganisms (higher than 25 % degradation occurred within 14 days).

 

Under the test conditions the percentage biodegradation of Morpholinium Sulphamate reached a mean of 7.3% after 28 days based on ThODNO3.Therefore the test item can be considered to be not ready biodegradable.

 

Biodegradation in water and sediment

The performance of simulation tests for biodegradation in water and sediment is scientifically unjustified.

REACH Regulation No. 1907/2006, Annex IX, Sect. 9.2.1.2, Col. 2, states as follows:

“9.2.1.2: The study need not to be conducted:

- if the substance is readily biodegradable, or

- if direct and indirect exposure of sediment is unlikely. ”

 

Direct and indirect exposure of the test item to water and sediment is unlikely. In addition, the substance is hydrolytically instable forming morpholine and sulphamic acid. Morpholine is readily biodegradable. Sulphamic acid is an anorganic compound which is not further biodegraded. Thus, further test on biodegradation are scientifically not justified.

Therefore simulation testing for biodegradation in sediment was considered not scientifically justified.

 

Biodegradation in soil

The performance of tests for biodegradation in soil is scientifically unjustified.

REACH Regulation No. 1907/2006, Annex IX, Sect. 9.2.1.3, Col. 2, states as follows:

“9.2.1.3: The study need not to be conducted:

- if the substance is readily biodegradable, or

- if direct and indirect exposure of soil is unlikely. ”

 

Direct and indirect exposure of the test item to soil is unlikely. In addition, the substance is hydrolytically instable forming morpholine and sulphamic acid. Morpholine is readily biodegradable. Sulphamic acid is an anorganic compound which is not further biodegraded. Thus, further test on biodegradation are scientifically not justified.

Therefore simulation testing for biodegradation in sediment was considered not scientifically justified.

 

Bioaccumulation

The performance of a test for bioaccumulation in aquatic species, preferably fish, is scientifically unjustified.

REACH Regulation No. 1907/2006, Annex IX, Sect. 9.3.2, Col. 2, states as follows:

“9.3.2 The study need not be conducted if:

- the substance has a low potential for bioaccumulation (for instance a log Kow <= 3) and/or a low potential to cross biological membranes, or

- direct and indirect exposure of the aquatic environment is unlikely. ”

 

In general, as shown in IUCLID5 section 4.8, the water solubility of morpholinium sulphamate is very good, the log Pow (IUCLID5 section 4.7) is clearly below 3, indicating that the substance has no bioaccumulation properties. Consequently, bioaccumulation in the environment does not have to be expected.

 

Adsorption / desorption

In accordance with column 2 adaptation statement of REACH Annex VIII and IX, adsorption/desorption screening and further studies on adsorption/desorption, information requirements 9.3.1 and 9.3.3, may be omitted since the log Kow value for the test substance is <3.0 (IUCLID section 4.7) and has low potential for adsorption. The log Kow for morpholinium sulphamate was determined to be -0.07.