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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Equivalent to GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1982
Report Date:
1982

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
GLP compliance:
yes
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Test material form:
solid: crystalline
Details on test material:
2-chloro-5-(trifluoromethyl) pyridine, a white crystalline solid, also know as CTF, was supplied by ICI Mond Division, CTL Ref Y00846/003/002

Test animals

Species:
rat
Strain:
other: Alderley Park
Sex:
male/female

Administration / exposure

Duration of exposure:
4 h
Concentrations:
25.3, 16.07, 7.75 mg/l
No. of animals per sex per dose:
5
Control animals:
yes

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LC50
Effect level:
ca. 19 mg/L air
Based on:
test mat.
Exp. duration:
4 h
Mortality:
Within two days of exposure, six animals died in the 25.3 mg/l group and seven animals died in the 16.07 mg/l group. No deaths occurred in the 7.75 mg/l group.
Clinical signs:
other: During exposure and immediately afterwards, all test animals exhibited signs of CNS depression, irritation and at higher levels, convulsions. Excessive urination was noted in some animals. The severity and duration of these effects was dose related.
Body weight:
All test animals lost weight following exposure, but those that survived began to gain weight at about the middle of the observation period. The overall weight gain particularly of the females, tended to be less than that of the controls.
Gross pathology:
The weights of test animals’ lungs were expressed as a percentage of the last bodyweight before death. The males of the 25.3 mg/l group, all animals in 16.07mg/l group and the males in the 7.75 mg/l group all had significantly higher lung weights than controls.

Upon examination, most of the animals from the 25.3 and 16.07 mg/l groups had haemorrhagic lungs; many had abnormal livers and other visceral abnormalities. Some of these observations were carried out as soon after death as possible, autolysis of tissues may have influenced the results. The animals from the 7.75 mg/l group had lung lesions only and these were approximately equivalent to those seen on the control animals.

Any other information on results incl. tables

Table 1: Results of atmosphere analysis

Group

Atmospheric concentration (mg/l)

1

25.3 ± 1.84

2

16.07 ±0.19

3

7.75 ± 0.35

Applicant's summary and conclusion

Interpretation of results:
harmful
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The four hour LC50 of 2-chloro-5-(trifluoromethyl) pyridine (CTF) in the rat is approximately 19 mg/l.
Executive summary:

The four hour LC50 of 2-chloro-5-(trifluoromethyl) pyridine (CTF) in the rat is approximately 19 mg/l. The lung, liver and central nervous system all appear to be target organs at high concentrations and deaths could have been caused by severe effects in one or more of these organs.