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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Performed 1984 to guidelines appropriate to that date
Considered scientifically valid and described in sufficient details

Data source

Reference
Reference Type:
publication
Title:
Lack of mutagenic activity of white spirit
Author:
B Gochet, C de Meester, A Lonard
Gh Deknudt
Year:
1984
Bibliographic source:
Int Arch Occup Environ Health ( 1984) 53:359-364

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
Version / remarks:
Performed before full validation of method type
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Reference substance name:
White mineral oil (petroleum)
EC Number:
232-455-8
EC Name:
White mineral oil (petroleum)
Cas Number:
8042-47-5
Molecular formula:
C15 - C50
IUPAC Name:
White mineral oil
Test material form:
liquid
Specific details on test material used for the study:
Test material described as < 15% aromatic linear and branched hydrocarbons, boiling up to 190 C
The boiling point indicated C10 - C20

Method

Species / strainopen allclose all
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Species / strain / cell type:
S. typhimurium TA 1538
Species / strain / cell type:
S. typhimurium, other: 1530
Metabolic activation:
with and without
Metabolic activation system:
59 mix ( 50 i/plate) from Aroclor 1254 pretreated rat livers
Test concentrations with justification for top dose:
Top dose 10 ul per plate used. Cytotoxicity was seen in preliminary work at 100 ul and in some replicates, also seen at 10 ul
Vehicle / solvent:
No for direct incorporation method
Pre-incubation replicates used ethanol as solvent,
Controls
Untreated negative controls:
yes
Negative solvent / vehicle controls:
not specified
True negative controls:
no
Positive controls:
other: Historical
Positive control substance:
not specified
Details on test system and experimental conditions:
Solvent used only for pre-incubation replicates

Results and discussion

Test resultsopen allclose all
Species / strain:
S. typhimurium TA 1535
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Vehicle controls validity:
not specified
Untreated negative controls validity:
valid
True negative controls validity:
not applicable
Positive controls validity:
other: historical
Species / strain:
S. typhimurium TA 1537
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Vehicle controls validity:
not specified
Untreated negative controls validity:
valid
True negative controls validity:
not applicable
Positive controls validity:
other: historical
Species / strain:
S. typhimurium TA 98
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Vehicle controls validity:
not specified
Untreated negative controls validity:
valid
True negative controls validity:
not applicable
Positive controls validity:
other: historical
Species / strain:
S. typhimurium TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Vehicle controls validity:
not specified
Untreated negative controls validity:
valid
True negative controls validity:
not applicable
Positive controls validity:
other: historical
Species / strain:
S. typhimurium TA 1538
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Vehicle controls validity:
not specified
Untreated negative controls validity:
valid
True negative controls validity:
not applicable
Positive controls validity:
other: historical
Species / strain:
S. typhimurium, other: 1530
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Vehicle controls validity:
not specified
Untreated negative controls validity:
valid
True negative controls validity:
not applicable
Positive controls validity:
other: historical
Additional information on results:
Bacterial strains were supplied by Dr Ames and results compared with historical positive results obtained for each strain

Applicant's summary and conclusion

Conclusions:
The work was part of primary research into low-aromatic hydrocarbons with the direct support of Dr Ames in the development of specific methods to invistigate reverse gene mation in bacterial cultures.