Reaction mass of 2- (palmitoylamino)ethyl acrylate and 2-(stearoylamino)ethyl acrylate

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source (i.e. manufacturer or supplier) and lot/batch number of test material: provided by Sponsor, Lot number 91112Y
- Purity.: 92.4%


STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: The test sample was stored in a dark storage place at room temperature，
- Stability and homogeneity of the test material in the vehicle/solvent under test conditions (e.g. in the exposure medium) and during storage: stability


TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing (e.g. warming, grinding): The test sample(50.1mg) was weighed with an electronic analytical balance and then filled up to10mL with tetrahydrofuran. This solution(1mL) was filled up to10mL with eluent for HPLC analysis to prepare a test item solution. Tetrahydrofuran(1mL) was filled up to 10mL with the eluent and the solution was used as a solvent blank.

Radiolabelling:

no

Test temperature:

25℃

Details on study design: HPLC method:

EQUIPMENT
- Apparatus: LC-2010CHr(Built-in ultraviolet and visible spectrophotometer) (Shimadzu)
- Type: High-performance liquid chromatograph(No.LC-138)
- Type, material and dimension of analytical (guard) column: Inertsil CN-3(150mmx 2.1mm L.D.， particle size 5um，GL Sciences)


MOBILE PHASES
Eluent ： Methanol/purified water (65/35v/v)

DETERMINATION OF DEAD TIME
Retention time of test item(min)

REFERENCE SUBSTANCES
- Identity: Formamide (75-12-7); NN-Dimethyl benzamide 611-74-5; Atrazine 1912-24-9; Linuron 330-55-2; 1，2，3-Trichlorobenzene 87-61-6; Phenanthrene 85-01-8; DDT 50-29-3





DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 2μl
- Quantity of reference substances: 2-10 μl


REPETITIONS
- Number of determinations: 2

EVALUATION
- Calculation of capacity factors k': k= (tR-t0)/t0
- Determination of the log Koc value: log Koc= a×log k+b

Type:

log Koc

Value:

>= 3.87 - <= 4.61 dimensionless

Temp.:

25 °C

Remarks on result:

other: pH : not specific

Details on results (HPLC method):

attachment

Validity criteria fulfilled:

yes

Conclusions:

The log Koc of the test item is 3.87-4.61.

Executive summary:

HPLC method (OECD121, GLP. RL1) was applied to estimation of the Adsorption Coefficient(Koc）on soil and on sewage sludge of the test item. The test item is a multi- constituent substance, and two peaks of HPLC used for Koc analysis. The log Koc of the test item is 3.87-4.61.

Description of key information

The log Koc of the test item is 3.87-4.61.

Key value for chemical safety assessment

Koc at 20 °C:

7 413