Registration Dossier
Registration Dossier
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EC number: 952-218-9 | CAS number: -
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- For the determination of the test concentrations with HPLC, the stock solution of the standards was not prepared using test medium:methanol (8:2; v:v) containing 10 mM ammonium acetate as given in the study plan but with water:acetonitrile (1:1; v:v). This deviation has no influence on the outcome of the test because the test item was completely soluble in the solvent used (water:acetonitrile (1:1; v:v)).
- Deviations:
- yes
- Remarks:
- water:acetonitrile (1:1; v:v) as a medium
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Trisodium bis[2-hydroxy-5-nitro-3-[[2-oxo-1-[(phenylamino)carbonyl]propyl]azo]benzenesulphonato(3-)]cobaltate(3-)
- EC Number:
- 289-068-2
- EC Name:
- Trisodium bis[2-hydroxy-5-nitro-3-[[2-oxo-1-[(phenylamino)carbonyl]propyl]azo]benzenesulphonato(3-)]cobaltate(3-)
- Cas Number:
- 85959-73-5
- Molecular formula:
- C32H22CoN8O16S2.3Na
- IUPAC Name:
- Trisodium bis[2-hydroxy-5-nitro-3-[[2-oxo-1-[(phenylamino)carbonyl]propyl]azo]benzenesulphonato(3-)]cobaltate(3-)
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- water:acetonitrile (1:1; v:v)
Test organisms
- Test organisms (species):
- Daphnia magna
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 18–22 °C
- pH:
- 6 - 9
- Dissolved oxygen:
- 8.6 – 9.6 mg/L
- Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 9.83 ng/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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