potassium disodium bis[2-hydroxy-5-nitro-3-[[2-oxo-1-[(phenylamino)carbonyl]propyl]azo]benzenesulphonato(3-)]cobaltate(3-)

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

ISO 8692 (Water Quality - Fresh Water Algal Growth Inhibition Test with Scenedesmus subspicatus and Selenastrum capricornutum)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Trisodium bis[2-hydroxy-5-nitro-3-[[2-oxo-1-[(phenylamino)carbonyl]propyl]azo]benzenesulphonato(3-)]cobaltate(3-)

EC Number:

289-068-2

EC Name:

Trisodium bis[2-hydroxy-5-nitro-3-[[2-oxo-1-[(phenylamino)carbonyl]propyl]azo]benzenesulphonato(3-)]cobaltate(3-)

Cas Number:

85959-73-5

Molecular formula:

C32H22CoN8O16S2.3Na

IUPAC Name:

Trisodium bis[2-hydroxy-5-nitro-3-[[2-oxo-1-[(phenylamino)carbonyl]propyl]azo]benzenesulphonato(3-)]cobaltate(3-)

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

Scenedesmus sp.

Details on test organisms:

TEST ORGANISM
- Common name: Desmodesmus subspicatus
- Age of inoculum (at test initiation): Inoculum culture was kept 3-4 days under conditions at which the test was performed.
- Method of cultivation: The strain culture was preinoculated from the stock solution and cultivated in flasks with the test medium on indirect daylight at laboratory temperature.
Algae inoculum for the test was sampled from exponentially growing inoculum culture.

ACCLIMATION
- Acclimation period:3-4 days
- Culturing media and conditions (same as test or not): same as test

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

72 h

Test conditions

Test temperature:

23±2°C

pH:

8.05

Nominal and measured concentrations:

The stock solution: 0.5 g/l
350, 300, 250, 200, 150, 100, 50 ml/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Testing mixtures were incubated in Erlenmeyers’ flasks 250 ml
- Type: closed (air-permeable plugs)
- Material, size, headspace, fill volume: The flasks were placed on a shaker under the lighting ramp and were incubated under continuous illumination and shaking for 72 hours.

Lighting: under continuous white light, 60 µE/(m2.s) do 120 µE/(m2.s)

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

87.9 mg/L

Basis for effect:

biomass

Effect concentrations 2

Key result

Duration:

72 h

Dose descriptor:

EC5

Effect conc.:

26.6 mg/L

Basis for effect:

biomass

Effect concentrations 3

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

151.9 mg/L

Basis for effect:

growth rate

Effect concentrations 4

Key result

Duration:

72 h

Dose descriptor:

EC5

Effect conc.:

32.5 mg/L

Basis for effect:

growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:

yes