Registration Dossier
Registration Dossier
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EC number: 952-218-9 | CAS number: -
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1995
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 1: Static Method)
- Deviations:
- not specified
- GLP compliance:
- yes
Test material
- Reference substance name:
- Trisodium bis[2-hydroxy-5-nitro-3-[[2-oxo-1-[(phenylamino)carbonyl]propyl]azo]benzenesulphonato(3-)]cobaltate(3-)
- EC Number:
- 289-068-2
- EC Name:
- Trisodium bis[2-hydroxy-5-nitro-3-[[2-oxo-1-[(phenylamino)carbonyl]propyl]azo]benzenesulphonato(3-)]cobaltate(3-)
- Cas Number:
- 85959-73-5
- Molecular formula:
- C32H22CoN8O16S2.3Na
- IUPAC Name:
- Trisodium bis[2-hydroxy-5-nitro-3-[[2-oxo-1-[(phenylamino)carbonyl]propyl]azo]benzenesulphonato(3-)]cobaltate(3-)
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Poecilia reticulata
- Details on test organisms:
- Details on test organisms
TEST ORGANISM
- Common name: Poecila reticulata
- Age at study initiation: 3 months
- Length at study initiation: 2,5-3,5 cm
- Feeding during test: no
- 10 fishies were used
ACCLIMATION
- Acclimation: yes in dilution water
- Acclimation conditions: same as test
- Feeding frequency: 24h before test without feeding
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
Test conditions
- Test temperature:
- 23±1°C
- pH:
- 8.05
- Nominal and measured concentrations:
- The stock solution: 0.5 g/l
1000, 800, 700, 600, 400, 200 ml/l - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass
- Type: open
- Material, size, headspace, fill volume: glass aquarium, volume of 10l
OTHER TEST CONDITIONS
- Photoperiod: daily light
The dilution water was prepared just before the test by admixing 2,5 mL of stored solutions A, B, C and D
into 1 000 mL of distilled water.
A. 294,0 mg CaCl2.2H2O in 1 L of distilled water
B. 123,3 mg MgSO4.7H2O in 1 L of distilled water
C. 63,0 mg NaHCO3 in 1 L of distilled water
D. 5,5 mg KCl in 1 L of distilled water
The stock solution: 0.1 g.L-1
- Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrations
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 304 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance was tested for acute toxicity on fish Poecilia reticulata. The result of the test shows LC50 96h > 100 mg/l.
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