2-Butanone, 1,3,4-trihydroxy-

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From February 27, 2018 to May 03, 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Aspect: viscous liquid
Lot No. : 17346.D
Purity : 79%
Water content: 21%
Expiration date: May 31, 2018

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage (Bois-de-Bay, Satigny, Switzerland) was used.
The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day.

Duration of test (contact time):

28 d

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

The method used is basically the one described under No. 301 F in the OECD Guidelines for Testing of Chemicals.

A measured volume of inoculated mineral medium, containing a known concentration of test substance as the nominal sole source of organic carbon, is stirred in a closed flask at a constant temperature (+ 1°C) for up to 28 days. Evolved carbon dioxide is absorbed in sodium hydroxide pellets. The consumption of oxygen is determined by measuring the pressure drop in the respirometer flask. The Biological Oxygen Demand (BOD), amount of oxygen taken up by the microbial population during biodegradation of the test chemical (corrected for uptake by blank inoculum, run in parallel) is expressed as a percentage of ThOD (Theoretical Oxygen Demand, calculated from the elemental composition, assuming that carbon is oxidized to carbon dioxide, hydrogen to water and nitrogen to ammonium, nitrite or nitrate).

Reference substance:

benzoic acid, sodium salt

Remarks:

Sodium Benzoate (purity 99%)

Test performance:

Everyday the oxygen consumption of each flask is recorded and correct temperature and stirring are checked.

Key result

Parameter:

% degradation (O2 consumption)

Value:

77

Sampling time:

28 d

Details on results:

(S)-1,3,4-trihydroxybutan-2-one undergoes 77% biodegradation after 28 days in the test conditions. The 10- day window criterion is also fulfilled (11% biodegradation on day 5 and 67% on day 15).

Results with reference substance:

Degradation of sodium benzoate exceeded 40% after 7 days and 65% after 14 days: the activity of the inoculum was thus verified (validity criterion).

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

(S)-1,3,4-trihydroxybutan-2-one undergoes 77% biodegradation after 28 days in the test conditions. The 10- day window criterion is also fulfilled (11% biodegradation on day 5 and 67% on day 15).

(S)-1,3,4-trihydroxybutan-2-one did not inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore considered to be non-toxic to the inoculum at the test concentration.

(S)-1,3,4-trihydroxybutan-2-one should be regarded as readily biodegradable according to this test.

Executive summary:

(S)-1,3,4-trihydroxybutan-2-one undergoes 77% biodegradation after 28 days in the test conditions. The 10- day window criterion is also fulfilled (11% biodegradation on day 5 and 67% on day 15).

(S)-1,3,4-trihydroxybutan-2-one did not inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore considered to be non-toxic to the inoculum at the test concentration.

(S)-1,3,4-trihydroxybutan-2-one should be regarded as readily biodegradable according to this test.

 

Description of key information

The method used is basically the one described under No. 301 F in the OECD Guidelines for Testing of Chemicals.

(S)-1,3,4-trihydroxybutan-2-one undergoes 77% biodegradation after 28 days in the test conditions. The 10- day window criterion is also fulfilled (11% biodegradation on day 5 and 67% on day 15).

 

(S)-1,3,4-trihydroxybutan-2-one did not inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore considered to be non-toxic to the inoculum at the test concentration.

 

(S)-1,3,4-trihydroxybutan-2-one should be regarded as readily biodegradable according to this test.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Type of water:

freshwater

Additional information

The method used is basically the one described under No. 301 F in the OECD Guidelines for Testing of Chemicals.

(S)-1,3,4-trihydroxybutan-2-one undergoes 77% biodegradation after 28 days in the test conditions. The 10- day window criterion is also fulfilled (11% biodegradation on day 5 and 67% on day 15).

 

(S)-1,3,4-trihydroxybutan-2-one did not inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore considered to be non-toxic to the inoculum at the test concentration.

 

(S)-1,3,4-trihydroxybutan-2-one should be regarded as readily biodegradable according to this test.