Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 254-227-7 | CAS number: 38970-72-8
Three independent experiments were performed. The cells were in these experiments incubated with the test item in a concentration range of 0.004 – 7.8 µM (2-fold dilution steps) in experiment 1 and of 0.090 – 7.8 µM (1.5-fold dilution steps) in experiments 2 and 3 for 48 hours ± 1 h. The activation of the ARE-dependent pathway was assessed by measuring the luminescence induction compared to the vehicle control. In addition, the viability was assessed with an MTT assay.
One deviation was reported:
One of the luminescence readings for the DMSO vehicle control (9050) was excluded from the analysis of the positive control in experiment 1 as an outlier, since the variation was with 22.4% > 20%. And one of the luminescence readings for the EtOH vehicle control (2435) was excluded from the analysis of the test item in experiment 3, since the variation was with 20.2% > 20%. Based on evaluation of the data with Dixon’s Q-test these values were clear outliers. Evaluation: After excluding this reading, still 17 vehicles were left for the calculations with a variation of 11% in experiment 1 and 3. Before excluding all other acceptation criteria were met. The exclusion was only affecting the positive control reading in experiment 1, which already met the acceptation criteria before exclusion. And in the third experiment had no influence on the positive result observed with the test item, since this was observed both before and after exclusion. Excluding these values has therefore no effect on the validity of the study.
This deviation was considered to not have impacted the overall integrity of the study or the interpretation of the study results and conclusions.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Close Do not show this message again