Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 254-227-7 | CAS number: 38970-72-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22 May to 05 July 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- 1992
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Appearance: Clear colourless liquid
Batch: 170918339
Purity: 97.2%
Solubility in water: Very low - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: municipal sewage treatment plant ('Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands)
- Storage conditions: The freshly obtained sludge was kept under continuous aeration until further treatment
- Pretreatment: Before use, the sludge was coarsely sieved (1 mm) and washed with mineral medium. After treatment the concentration of suspended solids (SS) was determined to be 3.5 g/L in the concentrated sludge as used for the test.
- Concentration of sludge: The magnetically stirred sludge was used as inoculum at the amount of 3 mL per litre of mineral medium, leading to a SS concentration of 10.5 mg/L. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 12 mg/L
- Based on:
- TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Tap-water purified by reverse osmosis (Milli- RO), mineral components, and inoculum
- Test temperature: 22 and 23°C
- pH: 7.6 to 7.7
- pH adjusted: Only for the toxicity control at the start of the test (adjusted using 1M HCl)
- Aeration of dilution water: Yes
- Continuous darkness: Yes
TEST SYSTEM
- Number of culture flasks/concentration: 2 bottles/test suspension; 2 bottles/inoculum blank control; 1 bottle/procedural control; 1 bottle/toxicity control
- Method used to create aerobic conditions: Synthetic air (CO2 < 1 ppm)
- Measuring equipment: CO2-absorbers
SAMPLING
- Sampling frequency: Titrations were made every second or third day during the first 10 days, and thereafter at least every fifth day until day 28, for the inoculum blank and test item. Titrations for the procedure and toxicity control were made over a period of at least 14 days. - Reference substance:
- acetic acid, sodium salt
- Remarks:
- CASRN 127-09-3
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 3
- Sampling time:
- 28 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 2
- Sampling time:
- 28 d
- Details on results:
- The relative biodegradation values calculated from the measurements performed during the test period revealed no biologically relevant biodegradation of the test item (3% and 2%, based on ThCO2).
The Theoretical CO2 production (ThCO2) of the test item was calculated to be 3.16 mg CO2/mg. - Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- 1,1’-(1,1,3-trimethylpropane-1,3-diyl)bis(cyclohexane) was not readily biodegradable under the conditions of the modified Sturm test presently performed (3% and 2%, based on ThCO2). In the toxicity control, the test item was found not to inhibit microbial activity.
Reference
Acceptability of the Test:
1. The reference item was biodegraded by at least 60% (actual result: 81%) within 14 days.
2. The difference of duplicate values for %-degradation of the test item was always less than 20 (actual result: ≤ 2%).
3. The total CO2 release in the blank at the end of the test did not exceed 40 mg/L (56.5 mg CO2 per 2 litres of medium, corresponding to 28.3 mg CO2/L).
4. The Inorganic Carbon content (IC) of the test item (suspension) in the mineral medium at the beginning of the test was less than 5% of the Total Carbon content (TC). Since the test medium was prepared in tap-water purified by reverse osmosis (Milli- RO water, carbon levels < 500 ppb)), IC was less than 5% of TC (mainly coming from the test item, 12 mg TOC/L).
Since all criteria for acceptability of the test were met, this study was considered to be valid.
Description of key information
1,1’-(1,1,3-trimethylpropane-1,3-diyl)bis(cyclohexane) was not readily biodegradable under the conditions of the modified Sturm test presently performed; 3% and 2% in 28 days based on ThCO2. The test item was found not to inhibit microbial activity.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
