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EC number: 701-281-9 | CAS number: -
All acceptance criteria for controls given in the EC Regulation were met:
· During the 72 hours incubation period the cell density in the control cultures increased by a factor of about 123, corresponding to about 7.0 generations.
· The mean coefficient of variation for section-by-section specific growth rates was 10.4 %.
· The coefficient of variation of average specific growth rates during the whole test period in replicate control cultures was 2.7 %.
A Pseudokirchneriella subcapitata growth inhibition test according to the EC regulation 761/2009 Part C.3 and theOECD-Guideline 201 (adopted by the Council on 23rdMarch 2006)was performed to determine the possible effects of "3-HYDROXY-2-(HYDROXMETHYL)-2-METHYLPROPIONALDEHYDE"on the growth of a unicellular green algal species.
Eight different concentrations of between nominal 0.4 and 500.0 mg per L nutrient medium, spaced by a factor of about 3, were tested against one concurrent negative control (nutrient medium only).
Three replicates were used for each test culture and six replicates for the control culture. The cell count of the algae was about 104cells/mL at the start of the exposure in each vessel.
In each vessel the cell density of the algae was determined 24, 48 and 72 hours after the onset of incubation and the pH was measured at the begin and at the end of the incubation period. Visual observations on the appearance of the algal cultures were made each time when the cell densities were determined.
Possible test substance effects were determined by comparison of the yield and of the growth rates.
At the start and at the end of the experiment, filtered samples of the cultures exposed to the test substance, of the control cultures and also of the blank with the highest concentration of the test substance, but no algae, were taken and immediately analysed in duplicate by HPLC.
based on the yield NOEC = 10.4 mg/L
based on the average growth rates NOEC = 10.4 mg/L
based on the yield EC50 = 16.8 mg/L
based on the average growth rates EC50 = 67.6 mg/L
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