A mixture of: trisodium (2,4(or 2,6 or 4,6)-bis(3,5-dinitro-2-oxidophenylazo)-5-hydroxyphenolato)(2(or 4 or 6)-(3,5-dinitro-2-oxidophenylazo)-5-hydroxy-4(or 2 or 6)-(4-(4-nitro-2-sulfonatoanilino)phenylazo)phenolato)ferrate(1-); trisodium bis(2,4(or 2,6 or 4,6)-bis(3,5-dinitro-2-oxidophenylazo)-5-hydroxyphenolato)ferrate(1-); trisodium (2,4(or 2,6 or 4,6)-bis(3,5-dinitro-2-oxidophenylazo)-5-hydroxyphenolato)(2(or 4 or 6)-(3,5-dinitro-2-oxidophenylazo)-5-hydroxy-4(or 2 or 6)-(4-nitro-2-sulfonatophenylazo)phenolato)ferrate(1-); trisodium (2,4(or 2,6 or 4,6)-bis(3,5-dinitro-2-oxidophenylazo)-5-hydroxyphenolato)(2(or 4 or 6)-(3,5-dinitro-2-oxidophenylazo)-5-hydroxy-4(or 2 or 6)-(3-sulfonatophenylazo)phenolato)ferrate(1-); disodium 3,3'-(2,4-dihydroxy-1,3(or 1,5 or 3,5)-phenylenediazo)dibenzenesulfonate

Long-term toxicity to fish

Description of key information

NOEC (21 d, Oncorhynchus mykiss) =  1.0 mg/L (geometric mean of measured conc.)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 mg/L

Additional information

The toxicity of test substance to Oncorhynchus mykiss was assessed over a period of 21 days under flow-through conditions. The test was conducted according to the OECD Guideline No. 204 (1984).

This study examined nominal test article concentrations in the range from nominal 0.05 to 4.0 mg/L which were spaced by a geometric progression factor of 3.

At the nominal test concentration of 0.44 mg/L the analysed concentrations of test item in the test medium varied in the range from 67.2 % to 73.9 % of the nominal value, at the next higher test concentration of nominal 1.33 mg/L in the range from 67.1 % to 80.1 %. At the highest test concentration of nominal 4.0 mg/L 86.3 % of nominal was found. At the lower test concentrations the measured test substance concentrations were mostly lower than 80 % of the nominal values, possibly due to adsorption of the test substance onto surfaces (glass walls of the aquaria, test organisms and their feces). Therefore all biological results are based on the mean measured test substance concentrations (calculated as mean of all measurements per test concentration): 0.3 mg/L at a nominal concentration of 0.44 mg/L, 1,0 mg/L at a nominal concentration of 1.33 mg/L, and 3.5 mg/L at a nominal concentration of 4.0 mg/L.

Per concentration, one group of ten fish each was exposed to the test concentrations and untreated test medium (control). No mortality, no significant clinical signs, and no significant influence on fish growth compared to the control were observed at the test concentrations up to and including 1.0 mg/L within 21 days of exposure. At the highest test concentration of 3.5 mg/L (nominal 4.0 mg/L), all fish were dead within the first 4 days of exposure.