Registration Dossier
Registration Dossier
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A mixture of: trisodium (2,4(or 2,6 or 4,6)-bis(3,5-dinitro-2-oxidophenylazo)-5-hydroxyphenolato)(2(or 4 or 6)-(3,5-dinitro-2-oxidophenylazo)-5-hydroxy-4(or 2 or 6)-(4-(4-nitro-2-sulfonatoanilino)phenylazo)phenolato)ferrate(1-); trisodium bis(2,4(or 2,6 or 4,6)-bis(3,5-dinitro-2-oxidophenylazo)-5-hydroxyphenolato)ferrate(1-); trisodium (2,4(or 2,6 or 4,6)-bis(3,5-dinitro-2-oxidophenylazo)-5-hydroxyphenolato)(2(or 4 or 6)-(3,5-dinitro-2-oxidophenylazo)-5-hydroxy-4(or 2 or 6)-(4-nitro-2-sulfonatophenylazo)phenolato)ferrate(1-); trisodium (2,4(or 2,6 or 4,6)-bis(3,5-dinitro-2-oxidophenylazo)-5-hydroxyphenolato)(2(or 4 or 6)-(3,5-dinitro-2-oxidophenylazo)-5-hydroxy-4(or 2 or 6)-(3-sulfonatophenylazo)phenolato)ferrate(1-); disodium 3,3'-(2,4-dihydroxy-1,3(or 1,5 or 3,5)-phenylenediazo)dibenzenesulfonate
EC number: 406-870-1 | CAS number: 115100-55-5
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
LC50 (96 h, Cyprinus carpio) = 3.52 mg/L (nominal)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 3.52 mg/L
Additional information
The short-term toxicity to fish was evaluated performing a study in accordance with the OECD guideline 203 (1984). Ten fish per concentration were exposed for 96 hours in a semi-static system to a range of nominally 1 to 10 mg/L forming a geometric progression with a factor of 1.8. The test media were renewed after 48 hours.
After 24 hours of exposure all fish had died at nominally 10 mg/L and 90 % mortality was recorded at 5.6 mg/l. After 96 hours of exposure the lowest concentration at which 100 % mortality was recorded still was 10 mg/L (LC100), with 90 % mortality at 5.6 mg/L and 40 % mortality at 3.2 mg/L. No mortality of fish was observed at and below nominally 1.8 mg/L (LC0).
Effects other than mortality, were observed in fish exposed to 3.2 mg/L and higher. The effects recorded were hypoactivity, loss of equilibrium and immobility. No effects were recorded in the fish exposed to 1.8 mg/L and lower.
Analysis of the samples taken at the start (t=0) of the final test revealed mean actual concentrations of > 95 % relative to the nominal concentrations of 1, 3.2 and 10 mg/L.
The LC50 value calculated at 96 hours was 3.52 mg/L, with a 95 % confidence interval of 3.07 to 4.44.
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