Registration Dossier
Registration Dossier
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A mixture of: trisodium (2,4(or 2,6 or 4,6)-bis(3,5-dinitro-2-oxidophenylazo)-5-hydroxyphenolato)(2(or 4 or 6)-(3,5-dinitro-2-oxidophenylazo)-5-hydroxy-4(or 2 or 6)-(4-(4-nitro-2-sulfonatoanilino)phenylazo)phenolato)ferrate(1-); trisodium bis(2,4(or 2,6 or 4,6)-bis(3,5-dinitro-2-oxidophenylazo)-5-hydroxyphenolato)ferrate(1-); trisodium (2,4(or 2,6 or 4,6)-bis(3,5-dinitro-2-oxidophenylazo)-5-hydroxyphenolato)(2(or 4 or 6)-(3,5-dinitro-2-oxidophenylazo)-5-hydroxy-4(or 2 or 6)-(4-nitro-2-sulfonatophenylazo)phenolato)ferrate(1-); trisodium (2,4(or 2,6 or 4,6)-bis(3,5-dinitro-2-oxidophenylazo)-5-hydroxyphenolato)(2(or 4 or 6)-(3,5-dinitro-2-oxidophenylazo)-5-hydroxy-4(or 2 or 6)-(3-sulfonatophenylazo)phenolato)ferrate(1-); disodium 3,3'-(2,4-dihydroxy-1,3(or 1,5 or 3,5)-phenylenediazo)dibenzenesulfonate
EC number: 406-870-1 | CAS number: 115100-55-5
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
ErC50 (72 h, Scenedesmus subspicatus)= 21.9 mg/L (nominal)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 21.9 mg/L
Additional information
The influence of test substance on the growth of the green alga Scenedesmus subspicatus was investigated. The test method was based on the OECD Guideline No. 201 (1984) and the EEC Directive 92/69 Part C.3, and was modified to differentiate between a reduced growth of algae due to direct inhibitory effects of the test article on the algal cells and a reduced growth of algae due to an indirect effect caused by the light absorption of the coloured test solutions.
The growth inhibition effect on Scenedesmus subspicatus was in part caused by the indirect effect, i.e. the light absorption of the coloured test solutions.
Nevertheless, especially at the highest test concentration of 100 mg/L, a direct inhibitory effect of test item on the algae growth cannot be excluded. Therefore, the results of experimental part in which only the direct inhibitory effect of substance is considered should be used in the assessment of the influence of test substance on the growth of Scenedesmus subspicatus.
At the beginning of the test (immediately after treatment), the actual test item mean measured concentrations were found to be in the range from 81 % to 100 % of nominal, and after 72 hours of exposure from 84 % to 95 % of nominal. Based on these results, the biological data are expressed as nominal concentrations.
The ErC50 (72 h, Scenedesmus subspicatus) was found to be 21.9 mg/L (nominal).
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