4-allylanisole

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-allylanisole

EC Number:

205-427-8

EC Name:

4-allylanisole

Cas Number:

140-67-0

Molecular formula:

C10H12O

IUPAC Name:

1-methoxy-4-(prop-2-en-1-yl)benzene

Specific details on test material used for the study:

Purity: 99.53%
Appearance: yellow to slight yellow clear liquid
Storage conditions: cool, dry place, protected from fire.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

As test system Daphnia magna Straus (Cladocera, Crustacea) was employed in the assay, as described by the guideline OECD 202:2004. The animals were less than 24 hours old at the start of the test, and they were not first brood progeny. They derived from Daphnia magna ephippia, supplied by the qualified provider ECOTOX LDS. Ephippia are a quiescent form of animals which can be hatched when needed.
The vials with the ephippia were stored in the refrigerator at 5 °C ± 2 °C; they were provided with a quality control certificate which reported the test species, the batch number, the expiration date, the storage and hatching conditions, the expected hatching, and the EC50 - 24 h value for K2Cr2O7. As internal quality control, EC50 value was determined for the batch of ephippia used in the test, and reported in the control chart Mod. 775/GxP.

Study design

Test type:

static

Total exposure duration:

48 h

Test conditions

pH:

7.4-7.9

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

17.583 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under these conditions, the EC50 for the test substance Estragole, batch n° 20161212 resulted 17.583 mg/l.