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EC number: 266-840-7
CAS number: 67656-24-0
Table 1: Validity criteria
Criterion from the guideline
Validity criterion fulfilled
The blank controls (without the test substance or reference substance) oxygen uptake rate should not be less than 20 mg oxygen per one gram of activated sludge (dry weight of suspended solids) in an hour.
21.3 mg oxygen per one gramm sludge per hour
The coefficient of variation of oxygen uptake rate in control replicates should not be more than 30% at the end of definitive test.
The EC50 of the reference item (inhibition of total respiration) must be within a range of 2 to
NOEC (3 h) = ≥ 1000 mg/L (nominal,
There is one GLP study available investigation the respiration
inhibition of activated sludge microorganisms according to OECD
guideline 209 (Hydrotox, 2017).
To investigate the inhibitory effects on total respiration, non-adapted
activated sludge from a municipal sewage treatment plant was fed with
OECD synthetic sewage and inoculated with the nominal concentration of
1000 mg/L substance for 3 h under aerobic conditions. Blank controls
(without test item) and sensitivity controls (with a reference item at
three concentration levels) were carried out in parallel. After 3 h, the
oxygen consumption rate was measured by inserting an oxygen electrode
into a BOD bottle and recording oxygen concentration for up to 15 min.
No respiration inhibition of the activated sludge compared to the blank
controls was determined up to 1000 mg/L test item. Therefore EC50 and
LOEC were reported as >1000 mg/L and the NOEC as ≥ 1000 mg/L.
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