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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
15 Aug – 13 Sep 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
adopted: 13 April 2004
Deviations:
no
GLP compliance:
yes (incl. certificate)
Remarks:
Landesanstalt für Umwelt, Messungen und Naturschutz Baden-Württenberg, Germany
Analytical monitoring:
yes
Details on sampling:
- Concentrations: Control and 100 mg/L
- Sampling method: 10 mL samples were taken at test start and test end (48h); All samples and the control were stabilized (1:1) with acetonitrile
- Sample storage conditions before analysis: All samples were stored deep frozen
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: test item was solved in test medium by shaking and test solutions were prepared by dilution of appropriate solutions with test medium.
- Controls: yes, medium only
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Strain/clone: Straus Clone V
- Feeding during test: no
- Food type: Single cell green algae (Desmodesmus subspicatus, formerly Scenedesmus subspicatus)
- Frequency: at least three times a week

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: Freshly hatched daphnids (not the first brood) less than 24 h old were used for the test
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
13°dH (232 mg/L as CaCO3)
Test temperature:
19.8 – 20.2 °C
pH:
7.77 – 7.99 (control)
7.78 - 8.07 (100 mg/L)
Dissolved oxygen:
7.8 - 9.0 mg/L (control)
8.6 - 9.1 mg/L (100 mg/L)
Nominal and measured concentrations:
Control and 100 mg/L (nominal)
Details on test conditions:
TEST SYSTEM
Test vessel:
- Type: open (covered with a glas plate)
- Material, size, headspace, fill volume: 100 ml glass vessel, filled with approx. 50 mL, 50 mL headspace
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: approx. 10 mL of test solution for each animal

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt medium was used as test medium (according to OECD 202)
- pH: 7.92
- Culture medium different from test medium: no
- Intervals of water quality measurement: Physical-chemcal parameters were measured at test start and after 24 and 48 h.

OTHER TEST CONDITIONS
- Photoperiod: 16 h photoperiod /8 h darkness daily
- Light intensity: 150 lux (mean at test start)

EFFECT PARAMETERS MEASURED: immobility at 24 and 48 h



Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Behavioural abnormalities: After 48 h of exposure no immobilisation was observed in all test item concentrations
- Mortality of control: After 48 h of exposure no immobilisation was observed in the control
- Other: Biological and analytical results are summarized within the tables 1-3 in section "Any other information on results incl tables".

Results with reference substance (positive control):
The results indicate an EC50 (24 h) of the reference item potassium dichromate around 1.00 mg/L (in accordance with the requirements of the OECD guideline 202).
Reported statistics and error estimates:
The NOEC was established based on the highest concentration at which the immobilisation is not higher than the allowed control immobilisation (≤10% immobilisation).

Table 1:  Determined concentration of Test Item

Test item

Sodium 2-butoxyethyl sulphate

Sampling

Test Item

nominal [mg/L]

nominal [mg/L]

[h]

[mg/L]

% of nominal

Control

0

0 fresh

n.d.

-

 

 

48 aged

n.d.

-

100

100

0 fresh

102

102

 

 

48 aged

106

106

 - = not calculated; n.d. = not detectable; LOQ = 1.00 mg/L Test Item

Table 2: Results of the test, 48 h values

 

Control

100 mg/L

 

Immobilised daphnids after 48 h

Group 1

0

0

Group 2

0

0

Group 3

0

0

Group 4

0

0

0

0

%

0

0

 

Table 3: Determined concentration of the test item

Test item

Test Item

Sampling

Test Item

nominal [mg/L]

nominal [mg/L]

[h]

[mg/L]

% of nominal

Control

0

0 fresh

n.d.

-

 

 

48 aged

n.d.

-

100

100

0 fresh

102

102

 

 

48 aged

106

106

 - = not calculated; n.d. = not detectable; LOQ = 1.00 mg/L Test item

Table 4: Validity criteria

Criterion from the guideline

Outcome

Validity criterion fulfilled

In the control, including the control containing the solubilising agent, not more that 10% of the daphnids should have been immobilized.

0%

yes

The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels.

≥ 7.8 mg/L

yes

Validity criteria fulfilled:
yes
Conclusions:
No effects were observed up to the test item concentration of 100 mg/L therefore the EC50 was determined to be at >100 mg/L and the NOEC at 100 mg/L.

Description of key information

EC50 (48h) >100 mg/L (Daphnia magna, OECD 202, nominal)

Key value for chemical safety assessment

Additional information

One study is available, in which the short term toxicity to invertebrates was investigated according to GLP and OECD guideline 202 (Eurofins, 2017b). The test was performed with a test item concentration of 100 mg/L in a static test system with the test organism Daphnia magna. The initial measured content of the test item was 102% of nominal and the aged measured content was 106% of nominal. Therefore the results are given based on nominal concentrations. Since no effects were observed the EC50 (48 h) was determined to be > 100 mg/L.