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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Link to relevant study record(s)

Description of key information

Long-term toxicity of NExBTL renewable diesel (a UVCB substance of similar composition to the substance) toward an aquatic invertebrate was determined in GLP compliant laboratory according to OECD Guidelines for Testing Chemicals (1998) no 211 "Daphnia magna, Reproduction test". The 14 and 21 Day EL50* (immobilisation) values for parental generation were estimated to be greater than 100 mg/l WAF. The 21 Day EL50* (reproduction) value was estimated to be greater than 100 m/l WAF. The LOEC was considered to be 3.2 mg/l WAF on the basis that at this loading rate significantly (p < 0.05) fewer youngs per adult were produced when compared to the control. The NOEC was considered to be 1 mg/l WAF on the basis that at this loading rate there were no significant mortalities (immobilisation) observed in the parental generation and that there were no significant (p > 0.05) differences between the control and the 1.0 mg/l loading rate WAF test group in terms of numbers of live young produced per adult by Day 21.

Supporting PETROTOX (version 4.01) chronic toxicity to aquatic invertebrates value is available. The model predicted an EL10 of 14.9 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
1 mg/L

Additional information

The aquatic toxicity was estimated using the PETROTOX (version 4.01) computer model, which combines a partitioning model (used to calculate the aqueous concentration of hydrocarbon components as a function of substance loading) with the Target Lipid Model (used to calculate acute and chronic toxicity of non-polar narcotic chemicals). PETROTOX computes toxicity based on the summation of the aqueous-phase concentrations of hydrocarbon block(s) that represent a petroleum substance and membrane-water partition coefficients (KMW) that describe the partitioning of the hydrocarbons between the water and organism.

The model predicted a chronic EL10 of 14.9 mg/L.