Reaction mass of Cardanol diene and Cardanol monoene and Cardanol triene 

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

no

Analytical monitoring:

not specified

Details on test solutions:

Preparation of the test solutions/dispersions : The test substance was slightly soluble in water. Therefore, the test solution was prepared by dissolving 800mg/L of the test substance in 1.8 liters of potable water (passed through reverse osmosis system) with continuous stirring of 48 hrs. After the completion was 48 hrs stirring, the sample was run with HPLC to calculate the actual solubility of substance and the solubility calculated was 1.4 mg/L. the water solubility obtained from the stock prepared is then used for achieving test concentrations of 0.02 mg/L, 0.04 mg/L, 0.06 mg/L, 0.08 mg/L& 0.2 mg/L, respectively. pH: 7.2

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM- Common name: Zebra Fish (Danio rerio)- Source: Orange City Aquarium, Sitabuldi, Nagpur, MS (India) - Age at study initiation (mean and range, SD): 3-4 months- Length at study initiation (length definition, mean, range and SD): average 2.3 cm- Weight at study initiation: 0.268 g (average) - Housing :The fishes were kept in a static tank in tap water, under natural conditions along with proper feed and aeration.- Duration of housing : 15 days - Photoperiod : 12 hours light and 12 hours darkness- Oxygen content : 7.4 (mg/l)- pH : 7.1- Water temperature : 28 °C- Mortality (%): During last 14 days = 0% mortality- Diet : Standard brand feed, once daily

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

38.75 (mg of CaCO3)

Test temperature:

21°C

pH:

7.2

Dissolved oxygen:

7.5 mg/l

Salinity:

Details not available

Nominal and measured concentrations:

0.02 mg/L, 0.04mg/L, 0.06 mg/L, 0.08 m/L, & 0.2 mg/L.

Details on test conditions:

TEST SYSTEM- Test vessel: The test vessels used were all plastic aquaria having the water capacity of 5 liters.- Aeration: Aeration in test vessels was provided during the experiment after 24 hours of the start of the experiment.

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 0.08 - < 0.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

A 96 h study was conducted for CAS No. 37330-39-5 on Zebra fish, at a test concentrations of 0.02 mg/L, 0.04mg/L, 0.06 mg/L, 0.08 m/L, & 0.2 mg/L. The fishes were moving slowly as compared to control. Mortality was found in the control aquaria. Mortality in each test concentration was nullified for the one mortality observed in control. LC0 (96 hours) (highest loading at which no mortality was observed) = Mortality was observed in all the five concentrationsLC50 (96 hours) Experimental = > 0.08 mg/L and <0.2 mg/LLC100 (96 hours) (lowest loading at which 100% mortality was observed) = 100% mortality was not in any of the concentration.

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No. 37330-39-5 on Zebra Fish was found to be > 0.08 mg/L and < 0.2 mg/L.

Executive summary:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for Reaction mass of Cardanol diene and Cardanol monoene and Cardanol triene. The nominal concentration selected for the experiment were 0.02 mg/L, 0.04 mg/L, 0.06 mg/L, 0.08 mg/L& 0.2 mg/L and Zebra fishes were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be > 0.08 mg/L and <0.2 mg/L.

Description of key information

             
Fish Acute Toxicity test according to OECD Guideline 203 was conducted forReaction mass of Cardanol diene and Cardanol monoene and Cardanol triene. The nominal concentration selected for the experiment were 0.02 mg/L, 0.04 mg/L, 0.06 mg/L, 0.08 mg/L& 0.2 mg/L and Zebra fishes were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be > 0.08 mg/L and <0.2 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.085 mg/L

Additional information

Short term toxicty to fish

Summary of the weight of evidence results is presented below:

The experimental study indicates the LC50 to range between >0.08 to <0.2 mg/L. The results of the 2 QSAR results are presented in the table below

Sr no	Endpoint	Effect values	Basis for effects	Duration	Species	Sources
1	LC50	20.21 mg/L	Mortality	96 hrs.	Oncorhynchus mykiss	Predicted data
2	LC50	10.30 mg/L	Mortality	96 hrs.	Platichthys flesus	Predicted data

However, the experimental study results shall be considered for the key value for the chemical safety assessment.