Registration Dossier
Registration Dossier
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EC number: 701-246-8 | CAS number: -
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
Pinene oligomers are not readily biodegradable (8% degradation after 28 days in OECD 301D test). Testing with a mixed and adapted inoculum (otherwise OECD 301B test method) found negligible biodegradation, but adsorption of test substance onto vessel walls prior to testing made bioavailability uncertain. The ready biodegradation test was repeated in accordance with OECD 301B due to limitations in the above testing and included enhancement measures alongside an inherent test (OECD 302C). Both tests indicated negligible degradation.
Read-across to the analogue limonene (readily biodegradable) and other considerations, including reports of extensive microbial metabolism of mixed phytoterpenes by marine microbes lead to a weight-of-evidence conclusion.
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable, not fulfilling specific criteria
- Type of water:
- freshwater
Additional information
Significant biodegradation of pinene oligomers has not been demonstrated. However limonene, a direct analogue of the monomer unit within pinene oligomers, is readily biodegradable. Other phytoterpene analogues are also known to be biodegradable and:
- these and other phytoterpenes of natural origin are ubiquitous in nature
- a variety of microorganisms are known to metabolise phytoterpenes.
It is concluded that pinene oligomers are not readily biodegradable and have not been shown to be degraded rapidly by an acclimated inoculum; however any pinene oligomers not rapidly removed from surface waters by adsorption onto particulates are likely to be slowly biodegraded.
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