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EC number: 257-581-0
CAS number: 51988-14-8
of the mutagenic activity of
performed according to OECD 471 under GLP in the Salmonella typhimurium
reverse mutation assay and the Escherichia coli reverse mutation assay
(plate incorporation and pre-incubation methods)
benzenecarbothioate was tested in the Salmonella typhimurium reverse
mutation assay with four histidine-requiring strains of Salmonella
typhimurium (TA1535, TA1537, TA100 and TA98) and in the Escherichia coli
reverse mutation assay with a tryptophan-requiring strain of Escherichia
coli (WP2uvrA). The test was performed in two independent experiments,
at first a direct plate assay was performed and secondly a
pre-incubation assay both in the absence and presence of S9-mix (rat
liver S9-mix induced Aroclor 1254).
study procedures described in this report were based on the most recent
OECD and EC guidelines.
vehicle of the test item was dimethyl sulfoxide.
the dose range finding study, the test item was initially tested up to
concentrations of 5000 µg/plate in the strains TA100 and WP2uvrA in the
direct plate assay. The test item precipitated on the plates at dose
levels of 512 or 1600 µg/plate and upwards. Cytotoxicity, as evidenced
by a decrease in the number of revertants and/or reduction of the
bacterial background lawn, was observed in both tester strains in the
absence and presence of S9-mix.
the first mutation experiment, the test item was tested up to
concentrations of 1600 µg/plate in the strains TA1535, TA1537 and TA98.
The test item precipitated on the plates at the dose level of 1600
µg/plate, except in tester strain TA1535. The bacterial background lawn
was not reduced at any of the concentrations tested and no biologically
relevant decrease in the number of revertants was observed.
the second mutation experiment, the test item was tested up to
concentrations of 1600 µg/plate in the tester strains TA1535, TA1537,
TA98, TA100 and WP2uvrA in the pre-incubation assay. The test item
precipitated on the plates at the dose level of 1600 µg/plate.
Cytotoxicity, as evidenced by a decrease in the number of revertants,
was only observed in tester strain TA98 in the absence of S9-mix.
negative and strain-specific positive control values were within the
laboratory historical control data ranges indicating that the test
conditions were adequate and that the metabolic activation system
test item did not induce a significant dose-related increase in the
number of revertant (His+) colonies in each of the four tester strains
(TA1535, TA1537, TA98 and TA100) and in the number of revertant (Trp+)
colonies in tester strain WP2uvrA both in the absence and presence of
S9-metabolic activation. These results were confirmed in an
independently repeated experiment.
on the results of this study it is concluded that the test item is not
mutagenic in the Salmonella typhimurium reverse mutation assay and in
the Escherichia coli reverse mutation assay.
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