3-methylpent-1-en-3-ol

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was performed in accordance to the German national test guideline DIN 38412, part 11, October 1982

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German standard DIN 38412, L15

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-methylbut-3-en-2-ol

EC Number:

204-068-4

EC Name:

2-methylbut-3-en-2-ol

Cas Number:

115-18-4

Molecular formula:

C5H10O

IUPAC Name:

2-methylbut-3-en-2-ol

Details on test material:

- Name of test material (as cited in study report): 2-methylbuten-3-ol-2
- Analytical purity: > 99 %
- Lot/batch No.: 88/70

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The product was added to the test water without any pretreatment; subsequently the fish were placed in to the aquaria. A control (test water without test substance) was tested in parallel. No undissolved test substance was visible in the aquaria.

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden orfe
- Length at study initiation (length definition, mean, range and SD): 7.7 cm (6.9-8.9 cm)
- Weight at study initiation (mean and range, SD): 4.3 g (2.8-6.6 g)
- Feeding during test: withdrawal of food 1 day before and during exposure
-Corpulence factor of the batch: 0.9
- Housing: Duration of housing: ca. 5 months Mortality during last 2 weeks of housing: ca. 1.0 % Medical treatment: twice with 0.05 mg/l malachite green chloride, once with 10 mg/l tetracycline hydrochloride Water temperature: 13 - 23°C
ACCLIMATION
- Acclimation period: 3 days
- Type and amount of food: growing feed (ad libitum);
- Health during acclimation (any mortality observed): 0.3 % Mortality

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

21 - 22°C

pH:

7.3 to 7.8

Dissolved oxygen:

7.2 to 8.8 mg/l

Nominal and measured concentrations:

Nominal test concentrations: control, 1000, 2150, 4640 and 10000 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: all-glass aquaria (30 * 22 * 24 cm) filled
- Fill volume: 10 l
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 4.3 g fish/l test water


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, Part II, 1982) that was prepared from fully demineralised tap water by addition of 294.0 mg/l CaCl2*2 H2O, 123.3 mg/l MgSO4*7 H2O, 63.0 mg/l NaHCO3 and 5.5 mg/l KCl. The test water had a total hardness of 2.5 mmol/l, an acid capacity of 0.8 mmol/l and a pH of ca. 8.0.
- Intervals of water quality measurement: after 1, 24, 48, 72 and 96 hours of exposure


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h (light:dark)

Reference substance (positive control):

yes

Remarks:

chloroacetamide

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

2 150 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 2 150 - 4 600 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

3 144.84 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: ; geom. mean

Effect concentrations 5

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

4 600 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

The LC50 (48 h) of the positive control chloroacetamide was about 26 mg/l, which corresponds to the normal sensitivity of the fish.

Any other information on results incl. tables

Sublethal observations / clinical signs:

During the 96 h exposure to 2-methylbut-3-en-2-ol, mortalities were as follows:

Nominal test cumulative mortality after
concentration 1 h 4 h 24 h 48 h 72 h 96 h
(mg/l)
Control 0 0 0 0 0 0
1000 0 0 0 0 0 0
2150 0 0 0 0 0 0
4640 0 100 100 100 100 100
10000 0 100 100 100 100 100

Maximum test concentration causing no mortality: 2150 mg/l.
Minimum test concentration causing 100% mortality: 4640 mg/l.

Symptoms of intoxication (apathy, narcotic-like state, tumbling) could be observed at nominal 2150 mg/L.

EC0 (96 h): 1000 mg/L; LC0 (96 h): 2150 mg/L

All values refer to nominal concentrations of 2-Methylbut-3-en-2-ol.
No chemical analysis of the test substance was carried out.