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Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: A single species (Pseudomonas putida) instead of activated sludge from a non-adapted source was used in the study. Scientifically acceptable.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Principles of method if other than guideline:
The Growth inhibition test according to Brinkmann & Kühn, 1986 was the precurser of the German national test guideline DIN 38412, part 8
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2-methylbut-3-en-2-ol
EC Number:
204-068-4
EC Name:
2-methylbut-3-en-2-ol
Cas Number:
115-18-4
Molecular formula:
C5H10O
IUPAC Name:
2-methylbut-3-en-2-ol
Details on test material:
- Name of test material (as cited in study report): 2-methylbuten-3-ol-2
no further details given

Sampling and analysis

Analytical monitoring:
no

Test solutions

Details on test solutions:
Concentration of stock solution: 12500 mg/L

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
- Laboratory culture: Pseudomonas putida, DSM 50026

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
17 h

Test conditions

Nominal and measured concentrations:
Nominal test concentrations: control, 2500, 5000, 7500 and 10000 mg/L.

Results and discussion

Effect concentrationsopen allclose all
Duration:
17 h
Dose descriptor:
EC10
Effect conc.:
6 260 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Duration:
17 h
Dose descriptor:
EC50
Effect conc.:
9 010 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Duration:
17 h
Dose descriptor:
other: EC90
Effect conc.:
> 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition

Any other information on results incl. tables

Change in the oxygen consuption rate of the test substance if compared to  the control:

test
concentration
(mg/L) % of control
5000 107.7
7500 72.5
10000 35.3

Applicant's summary and conclusion