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EC number: 210-236-8 | CAS number: 610-81-1
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
The biodegradation of the test item during the incubation time of 28 days was between 0% and 4.8% (ThIC). A degradation of the test item above the theorical threshold of 60% was never reached. Therefore, test item is not considered to be ready biodegradable.
Key value for chemical safety assessment
Additional information
The objective of the study was to assess the ready biodegradability of the test item in a CO2 headspace test over a period of 28 days, according to OECD 310 guideline and following the GLP. Activated sludge microorganisms from a domestic waste water treatment plant were supplied by a municipal sewage treatment plant. The biodegradation was determined by following the CO2 evolution of the test item in the incubation flasks during exposure. As a reference item sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control. The final sludge concentration in the flasks was 4 mg suspended solids/L. The test Item Loading Rate was 10.0 mg carbon per litre corresponding to 21.4 mg test item/L. Reference Item Loading Rate was 20.0 mg carbon per litre corresponding to 34.3 mg reference item /L. Test was conducted at 20°C - 21°C, in the darkness. The biodegradation of the test item during the incubation time of 28 days was between 0% and 4.8% (ThIC). A degradation above the threshold of 60% was never reached. Therefore, test item is not considered to be ready biodegradable. The reference item sodium benzoate was sufficiently degraded to 70.9% after 14 days and to 65.4% after 28 days of incubation. The percentage biodegradation of the reference item confirms the suitability of the used aerobic activated sludge inoculum.
In the toxicity control containing both, the test item and the reference item sodium benzoate, 47.3% biodegradation was noted within 14 days and 34.9% biodegradation after 28 days of incubation. Therefore it can be assumed that the test item is not inhibitory to the aerobic activated sludge microorganisms.
The mean amounts of TIC present in the blank controls at the end of the test was < 3 mg carbon per litre. All the validity criteria according to the OECD 310 guidelines are fulfilled.
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