Registration Dossier
Registration Dossier
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EC number: 210-236-8 | CAS number: 610-81-1
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
In an Amestest according to OECD guideline 471,4-amino-3-nitrophenol was weakly mutagenic in TA98 strain with and without metabolic activation and negative in all other strains tested.
In a mouse lymphoma assay according to OECD guideline 476 in cell line L5178Y (hprt-locus),the test substance was not mutagenic with and without metabolic activation.
In an in vitro chromosome aberration test according to OECD guideline 473,4-amino-3-nitrophenol induced chromosome aberrations in cultured human lymphocytes in the presence of S9-mix.
In an in vitro micronucleus test,the test substance induced micronuclei in cultured human peripheral blood lymphocytes following 3-h treatment in the presence of rat liver S9-mix (3+45 h) where treatment commenced 48 h following PHA (mitogen) stimulation.No effect was seen in the presence of S9-mix when treatment commenced 24 h after PHA stimulation.
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (positive)
Genetic toxicity in vivo
Description of key information
4-Amino-3-nitrophenol was evaluated as negative in the rat bone marrow micronucleus assay according to OECD guideline 474 under the conditions of this assay,when tested up to the testing limit (2000 mg/kg) for this assay which gave evidence of bone marrow toxicity.
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Additional information
Justification for classification or non-classification
While 4-amino-3-nitrophenol was was weakly mutagenic in the Ames test it was negative in a mouse lymphoma assay indicating that the substance does not possess a potential to cause gene mutation in vitro.
With metabolic activation 4-amino-3-nitrophenol revealed a potential to induce chromosome aberrations in cultured human lymphocytes and also induced micronuclei in cultured human peripheral blood lymphocytes.However,no evidence of a mutagenic/clastogenic potential was noted in vivo as the substance was negative in the rat bone marrow micronucleus assay.
The substance is therefore not classified.
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