Registration Dossier

Administrative data

Endpoint:
water solubility
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Experimental starting date: 28 April 2016 Experimental completion date: 20 April 2017
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report Date:
2017

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 105 (Water Solubility)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method A.6 (Water Solubility)
Deviations:
no
GLP compliance:
yes
Type of method:
flask method

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Test material form:
liquid
Specific details on test material used for the study:
Information as provided by the Sponsor. The Certificate of Analysis provided by the Sponsor is presented as Annex 1 (Please see Attachment Section of this Summary).

Identification: X-19574
Appearance/Physical state:Amber colored viscous liquid
Batch: X-19574-00-00
Purity: 100% (UVCB)
Expiry date: 01 June 2017
Storage conditions: Room temperature, in the dark

Results and discussion

Water solubilityopen allclose all
Key result
Water solubility:
0.021 g/L
Conc. based on:
test mat.
Loading of aqueous phase:
10 g/L
Incubation duration:
72 h
Temp.:
20 °C
pH:
6.3
Key result
Water solubility:
0.015 g/L
Conc. based on:
test mat.
Loading of aqueous phase:
1 g/L
Incubation duration:
72 h
Temp.:
20 °C
pH:
6.3

Any other information on results incl. tables

Results

 Preliminary Test

The preliminary estimate of water solubility was as follows:

Dibutylphosphorothioate: 2.08x 10-2g/L at 20.0 °C
C16 – C18 alkyl amines: 1.78 x 10-2g/L at 20.0 °C. 

Main Test

The mean peak areas relating to the standard and sample solutions are shown in the following tables:

Dibutylphosphorothioate

Solution

Mean peak area

Standard 5.26 mg/L

2.200 x 106

Standard 10.5 mg/L

4.231 x 106

Standard 21.0 mg/L

8.489 x 106

Standard 20.8 mg/L

8.577 x 106

Standard 52.6 mg/L

1.855 x 107

Standard 78.9 mg/L

2.551 x 107

Standard 105 mg/L

3.147 x 107

Standard 21.0 mg/L

9.985 x 106

Standard 20.8 mg/L

1.006 x 107

Sample 1A

8.12 x 106

Sample 1B

8.47 x 106

Sample 2A

5.23 x 106

Sample 2B

5.07 x 106

Sample 3A

8.50 x 106

Sample 3B

8.65 x 106

Sample 4A

6.63 x 106

Sample 4B

6.73 x 106

Sample 5A

9.82 x 106

Sample 5B

9.52 x 106

Sample 6A

5.91 x 106

Sample 6B

5.74 x 106

 


 C16 – C18 alkyl amines

Solution

Mean peak area

Standard 0.115 mg/L

4.700 x 105

Standard 0.574 mg/L

2.344 x 106

Standard 1.15 mg/L

4.475 x 106

Standard 2.30 mg/L

7.886 x 106

Standard 2.10 mg/L

7.501 x 106

Standard 5.74 mg/L

1.844 x 107

Standard 8.61 mg/L

2.748 x 107

Standard 11.5 mg/L

3.813 x 107

Standard 2.30 mg/L

8.411 x 106

Standard 2.10 mg/L

8.149 x 106

Sample 1A

3.92 x 106

Sample 1B

2.31 x 106

Sample 2A

8.60 x 106

Sample 2B

6.86 x 106

Sample 3A

2.01 x 106

Sample 3B

3.21 x 106

Sample 4A

9.83 x 106

Sample 4B

1.54 x 107

Sample 5A

3.47 x 106

Sample 5B

3.50 x 106

Sample 6A

6.86 x 106

Sample 6B

5.96 x 106

 

The concentration (mg/L) of test item in the sample solutions is shown in the following tables:

Dibutylphosphorothioate

Sample number

Loading rate (g/L)

Time shaken at

~ 30 ºC (hours)

Time equilibrated at 20 ºC (hours)

Concentration (mg/L)

Solution pH

A

B

Mean

1

10

24

24

18.0

18.9

18.5

6.3

2

1

24

24

10.8

10.4

10.6

6.3

3

10

48

24

19.0

19.4

19.2

6.2

4

1

48

24

14.2

14.5

14.4

6.4

5

10

72

24

22.4

21.6

22.0

6.4

6

1

72

24

12.4

12.2

12.3

6.3

Mean concentrations:

           Loading rate 10 g/L:  19.9 mg/L
           Loading rate 1 g/L:    12.4 mg/L

C16 – C18 alkyl amines

Sample number

Loading rate (g/L)

Time shaken at

~ 30 ºC (hours)

Time equilibrated at 20 ºC (hours)

Concentration (mg/L)

Solution pH

A

B

Mean

1

10

24

24

1.13

0.539

0.833

6.3

2

1

24

24

2.82

2.20

2.51

6.3

3

10

48

24

0.426

0.868

0.647

6.2

4

1

48

24

3.31

5.23

4.27

6.4

5

10

72

24

0.962

1.05

1.00

6.4

6

1

72

24

2.20

1.87

2.03

6.3

Mean concentrations:

           Loading rate 10 g/L:  0.828 mg/L
           Loading rate 1 g/L:    2.94 mg/L

Total Test Item

The water solubility in terms of total test item (dibutylphosphorothioateandC16 - C18 alkyl amines) for loading rates 10 and 1 g/L are shown below:

           Loading rate 10 g/L:              20.7 mg/L (2.07 x 10-2g/L)
           Loading rate 1 g/L:                15.4 mg/L (1.54 x 10-2g/L)


 

Total organic carbon analysis results are shown in the following table:

Total Organic Carbon

Sample number

Loading rate (g/L)

Time shaken at

~ 30 ºC (hours)

Time equilibrated at 20 ºC (hours)

TOC concentration (mg/L)

1

10

24

24

58.4

2

1

24

24

16.3

3

10

48

24

57.3

4

1

48

24

18.4

5

10

72

24

64.5

6

1

72

24

16.7

Mean TOC concentrations:

           Loading rate 10 g/L:  60.1 mg/L TOC, 100 mg/L as total test item
           Loading rate 1 g/L:    17.1 mg/L TOC, 28.5 mg/L as total test item

Applicant's summary and conclusion

Conclusions:
Conclusion
The water solubility of the test item has been determined to be as follows:


Test item loading rate (g/L) Mean water solubility, Total test item
(g/L at 20 °C)
10 2.07 x 10-2
1 1.54 x 10-2
Executive summary:

The determination was carried out using the flask method, designed to be compatible with Method A6 Water Solubility of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 105 of the OECD Guidelines for Testing of Chemicals, 27 July 1995.

The test system consisted of purified water with a resistivity of greater than 15 MΩ.

Conclusion

The water solubility of the test item has been determined to be as follows:

Test item loading rate
(g/L)

Mean water solubility, Total test item
(g/L at 20 °C)

10

2.07 x 10-2

1

1.54 x 10-2