Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report date:
2017

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Analysis of the test media samples was conducted on fresh media at 0 hours and on
corresponding old media at 48 hours

Test solutions

Vehicle:
no
Details on test solutions:
The definitive test was conducted at the following
nominal test substance concentrations: 30.7, 61.4, 123, 245 and 491 mg/L. The
491 mg/L treatment was pH adjusted prior to it being used to prepare the other test
concentrations. A control treatment was also included in the test.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Juvenile Daphnia magna (less than 24 hours old) were taken from healthy laboratory
cultures maintained in media of the same composition and quality as that used in the
toxicity tests. These cultures were setup originally from dormant eggs (ephippia)
supplied byMicroBioTests Inc., Belgium.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
Hardness (mg /L as CaCO3) 280
Test temperature:
19.4 – 20.7°C
pH:
7.44 - 7.73
Dissolved oxygen:
91 - 101%
Salinity:
Alkalinity (mg /L as CaCO3) 127
Conductivity:
639 - 986 (μS/cm)
Nominal and measured concentrations:
The measured test substance concentrations were between 66% – 122 % of nominal
test substance concentrations at 0 hours and 101 % - 120 % of nominal concentrations
at 48 hours.

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 592 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 592 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes