Registration Dossier
Registration Dossier
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EC number: 257-848-1 | CAS number: 52320-66-8
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from Authoritative database.
Data source
Reference
- Reference Type:
- other: Authoritative database.
- Title:
- Acute Dermal toxicity study of Pigment Yellow 97 (CAS no: 12225-18-2) in Rabbit.
- Author:
- U.S. National Library of Medicine
- Year:
- 2 017
- Bibliographic source:
- ChemIDplus, A TOXNET DATABASE, Lite Browse Advanced, 2017.
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: As mentioned below
- Principles of method if other than guideline:
- The acute Dermal toxicity test of N-(4-chloro-2,5-dimethoxyphenyl)-2-[[2,5-dimethoxy-4-[(phenylamino)sulphonyl]phenyl]azo]-3-oxobutyramide (CAS no: 12225-18-2) in rabbit.
- GLP compliance:
- not specified
- Test type:
- other: not specified
- Limit test:
- no
Test material
- Reference substance name:
- N-(4-chloro-2,5-dimethoxyphenyl)-2-[[2,5-dimethoxy-4-[(phenylamino)sulphonyl]phenyl]azo]-3-oxobutyramide
- EC Number:
- 235-427-3
- EC Name:
- N-(4-chloro-2,5-dimethoxyphenyl)-2-[[2,5-dimethoxy-4-[(phenylamino)sulphonyl]phenyl]azo]-3-oxobutyramide
- Cas Number:
- 12225-18-2
- Molecular formula:
- C26H27ClN4O8S
- IUPAC Name:
- N-(4-chloro-2,5-dimethoxyphenyl)-2-[[2,5-dimethoxy-4-[(phenylamino)sulphonyl]phenyl]azo]-3-oxobutyramide
- Details on test material:
- Name: N-(4-chloro-2,5-dimethoxyphenyl)-2-[[2,5-dimethoxy-4-[(phenylamino)sulphonyl]phenyl]azo]-3-oxobutyramide
InChI:1S/C26H27ClN4O8S/c1-15(32)25(26(33)28-18-12-20(36-2)17(27)11-21(18)37-3)30-29-19-13-23(39-5)24(14-22(19)38-4)40(34,35)31-16-9-7-6-8-10-16/h6-14,25,31H,1-5H3,(H,28,33)/b30-29+
Smiles: c1(c(cc(S(Nc2ccccc2)(=O)=O)c(c1)OC)OC) \N=N\[C@@H](C(Nc1c(cc(Cl)c(c1)OC)OC)=O)C(C)=O
- Name of test material (as cited in study report): Pigment Yellow 97
- Molecular formula (if other than submission substance):C26H27ClN4O8S
- Molecular weight (if other than submission substance):591.038 g/mol
- Substance type:Organic
Constituent 1
- Specific details on test material used for the study:
- Name: N-(4-chloro-2,5-dimethoxyphenyl)-2-[[2,5-dimethoxy-4-[(phenylamino)sulphonyl]phenyl]azo]-3-oxobutyramide
InChI:1S/C26H27ClN4O8S/c1-15(32)25(26(33)28-18-12-20(36-2)17(27)11-21(18)37-3)30-29-19-13-23(39-5)24(14-22(19)38-4)40(34,35)31-16-9-7-6-8-10-16/h6-14,25,31H,1-5H3,(H,28,33)/b30-29+
Smiles: c1(c(cc(S(Nc2ccccc2)(=O)=O)c(c1)OC)OC) \N=N\[C@@H](C(Nc1c(cc(Cl)c(c1)OC)OC)=O)C(C)=O
- Name of test material (as cited in study report): Pigment Yellow 97
- Molecular formula (if other than submission substance):C26H27ClN4O8S
- Molecular weight (if other than submission substance):591.038 g/mol
- Substance type:Organic
Test animals
- Species:
- rabbit
- Strain:
- not specified
- Sex:
- not specified
- Details on test animals or test system and environmental conditions:
- not specified
Administration / exposure
- Type of coverage:
- other: Dermal
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- not specified
- Duration of exposure:
- not specified
- Doses:
- 3000 mg/kg
- No. of animals per sex per dose:
- not specified
- Control animals:
- not specified
- Details on study design:
- not specified
- Statistics:
- not specified
Results and discussion
- Preliminary study:
- not specified
Effect levels
- Sex:
- not specified
- Dose descriptor:
- LD50
- Effect level:
- > 3 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: No mortality was observed.
- Mortality:
- No mortality was observed at 3000 mg/kg bw.
- Clinical signs:
- other: not specified
- Gross pathology:
- not specified
- Other findings:
- not specified
Applicant's summary and conclusion
- Interpretation of results:
- other: Not classified
- Conclusions:
- The acute Dermal LD50 value was considered to be >3000 mg/kg bw, when rabbits were treated with N-(4-chloro-2,5-dimethoxyphenyl)-2-[[2,5-dimethoxy-4-[(phenylamino)sulphonyl]phenyl]azo]-3-oxobutyramide (CAS no: 12225-18-2) by dermal application to the skin.
- Executive summary:
Acute Dermal toxicity study of N-(4-chloro-2,5-dimethoxyphenyl)-2-[[2,5-dimethoxy-4-[(phenylamino)sulphonyl]phenyl]azo]-3-oxobutyramide (CAS no: 12225-18-2) was conducted in rabbits at the concentration of 3000 mg/kg bw. No mortality was observed in treated rabbits at 3000 mg/kg bw. Therefore, LD50 was considered to be >3000 mg/kg bw, when rabbits were treated with N-(4-chloro-2,5-dimethoxyphenyl)-2-[[2,5-dimethoxy-4-[(phenylamino)sulphonyl]phenyl]azo]-3-oxobutyramide by dermal application to the skin.
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