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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Study period:
From March 17, 1999 to March 19, 1999
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
RA study
Reason / purpose:
read-across: supporting information
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
yes
Remarks:
TOC determination
Details on sampling:
Analysis of the only test concentration plus a control at start and after 48 h of exposure
Vehicle:
no
Remarks:
direct weighing
Test organisms (species):
Daphnia magna
Details on test organisms:
Species: Daphnia magna STRAUS, parthenogenetic females
Origin of the species: cloned at the Federal Health Office (BGA) in Berlin
Test animals: laboratory bred at Bayer AG Lever-kusen
Age: 0 - 24 h
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
15.1 °dH
Test temperature:
20.3 - 20.7
pH:
7.8
Dissolved oxygen:
8.1 mg/L O2
Nominal and measured concentrations:
0 and 100 mg/L (measured)
Details on test conditions:
Cylindrical test vessels: diameter 4.0 cm, height 6.5 cm
10 daphnids per vessel (in duplicate)
20 mL test medium
Not ventilated
Observations: mobility after 24 and 48 h
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
>= 80 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Remarks:
80 mg/L of the organic components is equivalent to 98 mg/L of the test substance including inorganic components
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
No effects observed
Results with reference substance (positive control):
-
Validity criteria fulfilled:
yes
Conclusions:
Under the study conditions, the mean measured 48 h EC0 of the substance in Daphnia magna was determined to be greater than 80 mg/L of the organic components (equivalent to 98 mg/L of the substance including inorganic components). The EC50 could not be determined due to absence of any adverse effects.
Executive summary:

A study was conducted to determine the short-term toxicity of the read-across substance to aquatic invertebrates according to EU Method C.2, in compliance with GLP. Daphnia magna were exposed for 48 h to the test substance at concentrations of 0 and 100 mg/L. Mobility was assessed daily. Analytical verification of test concentrations was conducted by TOC determination. There were no immobile daphnids in the test group. Under the study conditions, the mean measured 48 h EC0 of the substance in Daphnia magna was determined to be greater than 80 mg/L of the organic components (equivalent to 98 mg/L of the test substance including inorganic components) (Caspers, 1999). The EC50 could not be determined due to absence of any adverse effects.

Description of key information

Under the read-across study conditions, the mean measured 48 h EC0 of the substance in Daphnia magna was determined to be greater than 80 mg/L of the organic components (equivalent to 98 mg/L of the test substance including inorganic components). The EC50 could not be determined due to absence of any adverse effects.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
100 mg/L

Additional information

A study was conducted to determine the short-term toxicity of the read-across substance to aquatic invertebrates according to EU Method C.2, in compliance with GLP. Daphnia magna were exposed for 48 h to the test substance at concentrations of 0 and 100 mg/L. Mobility was assessed daily. Analytical verification of test concentrations was conducted by TOC determination. There were no immobile daphnids in the test group. Under the study conditions, the mean measured 48 h EC0 of the substance in Daphnia magna was determined to be greater than 80 mg/L of the organic components (equivalent to 98 mg/L of the test substance including inorganic components) (Caspers, 1999). The EC50 could not be determined due to absence of any adverse effects.