Trisodium 7-[[4-chloro-6-[ethyl[3-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]-4-hydroxy-3-[(4-methoxy-2-sulphonatophenyl)azo]naphthalene-2-sulphonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

From March 17, 1999 to March 19, 1999

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

RA study

Reason / purpose for cross-reference:

read-across: supporting information

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Remarks:

TOC determination

Details on sampling:

Analysis of the only test concentration plus a control at start and after 48 h of exposure

Vehicle:

no

Remarks:

direct weighing

Test organisms (species):

Daphnia magna

Details on test organisms:

Species: Daphnia magna STRAUS, parthenogenetic females
Origin of the species: cloned at the Federal Health Office (BGA) in Berlin
Test animals: laboratory bred at Bayer AG Lever-kusen
Age: 0 - 24 h

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

15.1 °dH

Test temperature:

20.3 - 20.7

pH:

7.8

Dissolved oxygen:

8.1 mg/L O2

Nominal and measured concentrations:

0 and 100 mg/L (measured)

Details on test conditions:

Cylindrical test vessels: diameter 4.0 cm, height 6.5 cm
10 daphnids per vessel (in duplicate)
20 mL test medium
Not ventilated
Observations: mobility after 24 and 48 h

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 80 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Remarks:

80 mg/L of the organic components is equivalent to 98 mg/L of the test substance including inorganic components

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

No effects observed

Results with reference substance (positive control):

-

Validity criteria fulfilled:

yes

Conclusions:

Under the study conditions, the mean measured 48 h EC0 of the substance in Daphnia magna was determined to be greater than 80 mg/L of the organic components (equivalent to 98 mg/L of the substance including inorganic components). The EC50 could not be determined due to absence of any adverse effects.

Executive summary:

A study was conducted to determine the short-term toxicity of the read-across substance to aquatic invertebrates according to EU Method C.2, in compliance with GLP. Daphnia magna were exposed for 48 h to the test substance at concentrations of 0 and 100 mg/L. Mobility was assessed daily. Analytical verification of test concentrations was conducted by TOC determination. There were no immobile daphnids in the test group. Under the study conditions, the mean measured 48 h EC0 of the substance in Daphnia magna was determined to be greater than 80 mg/L of the organic components (equivalent to 98 mg/L of the test substance including inorganic components) (Caspers, 1999). The EC50 could not be determined due to absence of any adverse effects.

Description of key information

Under the read-across study conditions, the mean measured 48 h EC0 of the substance in Daphnia magna was determined to be greater than 80 mg/L of the organic components (equivalent to 98 mg/L of the test substance including inorganic components). The EC50 could not be determined due to absence of any adverse effects.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

A study was conducted to determine the short-term toxicity of the read-across substance to aquatic invertebrates according to EU Method C.2, in compliance with GLP. Daphnia magna were exposed for 48 h to the test substance at concentrations of 0 and 100 mg/L. Mobility was assessed daily. Analytical verification of test concentrations was conducted by TOC determination. There were no immobile daphnids in the test group. Under the study conditions, the mean measured 48 h EC0 of the substance in Daphnia magna was determined to be greater than 80 mg/L of the organic components (equivalent to 98 mg/L of the test substance including inorganic components) (Caspers, 1999). The EC50 could not be determined due to absence of any adverse effects.