Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 805-172-8 | CAS number: 65646-25-5
Endpoint summary
Administrative data
Description of key information
Key value for chemical safety assessment
Skin sensitisation
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (sensitising)
- Additional information:
The study was performed to assess the skin sensitization potential of CHP using the local lymph node assay (LLNA).
Preliminary investigations were performed at 50% v/v and the test substance as supplied with 2 mice per concentration to establish the highest concentration of test substance which did not lead to systemic toxicity or excessive local irritation. The results of preliminary investigations indicated that the test substance as supplied would be a suitable high concentration for use on the main study.
The study comprised three treated groups, each comprising four female mice receiving CHP at concentrations of 25, 50% v/v or as supplied. Similarly constituted groups were either sham dosed, received the vehicle 4:1 v/v acetone:olive oil (AOO) or positive control substance (25% v/v hexyl cinnamic aldehyde). The mice were treated by daily application of 25 µL of the appropriate concentration or control (vehicle or positive), to the dorsal surface of both ears for three consecutive days. The proliferative response of the lymph node cells (LNC) from the draining auricular lymph nodes was assessed five days following the initial application, by measurement of the incorporation of 3H-methyl Thymidine (3HTdR) by ß-scintillation counting of LNC suspensions. The response was expressed as radioactive disintegrations per minute per lymph node (dpm/node) and as the ratio of 3HTdR incorporation into LNC of test nodes relative to that recorded for control nodes (test/control ratio), termed as Stimulation Index (SI). The test substance is regarded as a sensitizer if at least one concentration of the chemical has a SI of three or more.
Results
The SI results obtained for 25, 50% v/v and as supplied were 2.1, 1.1 and 5.5 respectively which indicates that CHP showed the potential to induce skin sensitization. The SI for the positive control substance hexyl cinnamic aldehyde was 6.3, which demonstrates the validity of this study.
Conclusion
CHP is regarded as a potential skin sensitizer.
Migrated from Short description of key information:
The SI results obtained for 25, 50% v/v and as supplied were 2.1, 1.1 and 5.5 respectively which indicates that CHP showed the potential to induce skin sensitization. CHP is regarded as a potential sensitizer
Justification for classification or non-classification
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
