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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
11 April 2016 - 05 July 2016
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Remarks:
gas chromatography (GC)
Details on sampling:
The test samples were thawed with the aid of sonication. The test samples (100 mL) plus 30 g of sodium chloride were extracted with 100 mL of hexane . An aliquot of the hexane layer was taken for analysis.
Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna is a freshwater invertebrate representative of a wide variety of natural habitats, and can therefore be considered as an important non-target organism in freshwater ecosystems.
Test type:
static
Water media type:
other:
Remarks:
Reconstituted water (ISO medium) used for both the range-finding and definitive tests
Limit test:
no
Total exposure duration:
48 h
Hardness:
approximate theoretical total hardness of 250 mg/L
Test temperature:
maintained at 21 °C to 22 °C
pH:
0 hours: 7.6; 48 hours: 7.9-8.0
Dissolved oxygen:
0 hours (mg O2/L): Control:7.9 ; 10%v/v: 7.3; 18%v/v: 7.3; 32%v/v: 7.4; 56%v/v: 7.8; 100%v/v: 8.6
48 hours (mg O2/L): Control:8.9 ; 10%v/v: 8.6; 18%v/v: 8.6; 32%v/v: 8.6; 56%v/v: 8.6; 100%v/v: 8.6
Nominal and measured concentrations:
10, 18, 32, 56 and 100% v/v saturated solution for 48 hours
After 24 hours: 10, 1.0 and 0.10% v/v saturated solution
Details on test conditions:
photoperiod of 16 hours light (between 200 and 1200 Lux) and 8 hours darkness with 20 minute dawn and dusk transition periods.
Reference substance (positive control):
yes
Remarks:
The results from the positive control with potassium dichromate were within the normal range for this reference item.
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
19 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other:
Remarks:
95% Confidence limits (mg/L):17-21
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
13 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
24 h
Dose descriptor:
NOEC
Effect conc.:
14 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
6.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
24 h
Dose descriptor:
LOEC
Effect conc.:
26 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
14 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
Sub-lethal effects of exposure were observed in the 14 mg/L test concentration. This response was reduced mobility at both 24 and 48 hours.
Results with reference substance (positive control):
The results from the positive control with potassium dichromate were within the normal range for this reference item.
Validity criteria fulfilled:
yes
Conclusions:
Exposure of Daphnia magna to the test item has been investigated and gave the following results based on the geometric mean measured test concentrations:

Time Point (Hours)- 48        
EC50 (mg/L)- 13            
95% Confidence Limits (mg/L)- Not determined
No Observed Effect Concentration (NOEC) (mg/L)-6.8         
Lowest Observed Effect Concentration (LOEC) (mg/L)-14





Description of key information

Exposure of Daphnia magna to the test item has been investigated and gave the following results based on the geometric mean measured test concentrations:

Time Point (Hours)- 48        

EC50 (mg/L)- 13            

95% Confidence Limits (mg/L)- Not determined

No Observed Effect Concentration (NOEC) (mg/L)-6.8         

Lowest Observed Effect Concentration (LOEC) (mg/L)-14

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
13 mg/L

Additional information