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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
11.5.2000 - 29.6.2000
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions
Qualifier:
according to guideline
Guideline:
other: Test No. L 47-Fish, Acute Toxicity test. Chemical formulation and waste waters
Deviations:
no
GLP compliance:
no
Analytical monitoring:
no
Test organisms (species):
Poecilia reticulata
Details on test organisms:
TEST ORGANISM
- Common name: Poecila reticulata
- Source: AKVA-SALON, 503 02 Neděliště 51
- Age at study initiation: 3-4 months
- Length at study initiation: 2-3 cm
- Feeding during test: no


ACCLIMATION
- Acclimation: yes
- Acclimation conditions: same as test
- Feeding frequency: 72h before test without feeding

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Test temperature:
22.0 ± 1.0 °C
pH:
7.8
Nominal and measured concentrations:
100.2 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass aquarium, volume of 10 L
- Type: Open
- Aeration: No
The dilution water was prepared just before the test by admixing 2.5 mL of stored solutions A, B, C and D into 1 000 mL of distilled water.
A. 117.6 g CaCl2.2H2O in 1 L of distilled water
B. 49.3 g MgSO4.7H2O in 1 L of distilled water
C. 25.9 g NaHCO3 in 1 L of distilled water
D. 2.3 g KCl in 1 L of distilled water

The stock solution: 0.1 g/L
The tested concentration: 100.2 mg/L
The volume of the test solution was 1 000 in the preliminary test and 4 000 mL in the limit test.

Preliminary test: 3 ks animals (in the one beaker – no replicates)
Verification test: 10 ks animals (in the one beaker – no replicates)
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Basis for effect:
mortality (fish)
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Basis for effect:
mortality (fish)
Details on results:
- Behavioural abnormalities: not observed
- Observations on body length and weight: not observed
- Other biological observations:not observed
- Mortality of control: not observed


The measured values of pH solution should not change more than 1 pH unit.
Validity criteria fulfilled:
yes
Remarks:
The measured values of pH solution should not change more than 1 pH unit.
Conclusions:
The test substance, Reactive red 45:1, was tested for acute toxicity on fish Poecilia reticulata The test was performed according to method est No. L 47-Fish, Acute Toxicity test. Chemical formulation and waste waters. The test was performed as static. No mortality was observed.

Description of key information

Reactive Red 45:1 caused no mortality in Poecilia reticulata at 100 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Dose descriptor:
LC50
Effect concentration:
> 100 mg/L

Additional information

The test substance, Reactive Red 45:1, was tested for acute toxicity on fish Poecilia reticulata. The test was performed according to method No. L 47-Fish, Acute Toxicity test. Chemical formulation and waste waters. The test was performed as static. No mortality was observed at 100 mg/L.

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