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Ecotoxicological information

Toxicity to microorganisms

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Reference
Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1986-1987
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Principles of method if other than guideline:
The test material is evaluated at selected concentrations in a mixture containing buffer, nutrients, growth substrate and microorganisms. The
“degree“ of inhibition can be assessed from measuring (optical density at 530 nm) the turbidity levels of the test material at various concentrations.
The measured optical density values are calculated as a percentage of the seeded growth control system.
GLP compliance:
no
Specific details on test material used for the study:
- Name of test material: Diisobutyl ketone
- Analytical purity: not stated
Analytical monitoring:
no
Test organisms (species):
aerobic microorganisms
Test type:
static
Water media type:
freshwater
Total exposure duration:
16 h
Details on test conditions:
TEST SYSTEM
- Test vessel: an 8-ounce, narrow—neck, round bottle

Duration:
16 h
Dose descriptor:
IC50
Effect conc.:
255 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Validity criteria fulfilled:
not specified
Conclusions:
A 16-hour IC50 of 255 mg/L was reported.
Executive summary:

Growth inhibition during a 16 hour exposure of aerobic microorganisms to diisobutyl ketone was reported. The 16 -hour IC50 was 255 mg/L.

Description of key information

Growth inhibition during a 16 hour exposure of aerobic microorganisms to diisobutyl ketone was reported.  The 16 -hour IC50 was 255 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:
255 mg/L

Additional information

Growth inhibition during a 16 hour exposure of aerobic microorganisms to diisobutyl ketone was reported. The 16 -hour IC50 was 255 mg/L.