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REACH

Tetraethylammonium chloride

EC number: 200-267-5 | CAS number: 56-34-8

Life Cycle description
Uses advised against

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

Study was conducted to access the effect of test chemical Tetraethylammonium chloride (CAS No.56-34-8) on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).

GLP compliance:

Analytical monitoring:

Vehicle:

Details on test solutions:

The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous stirring. This test solution was then added to the remaining three liters of water for achieving test concentrations of 6.25, 12.5, 25, 50 and 100 mg/L.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Unique Aqua Fish Corner
- Length at study initiation (length definition, mean, range and SD): average ----- cm
- Weight at study initiation (mean and range, SD): average ------- g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: ----------
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Hardness:

155.5 mg of CaCO3

Test temperature:

23 °C

pH:

7.2

Dissolved oxygen:

7.6 (mg/l)

Nominal and measured concentrations:

6.25, 12.5 25, 50 and 100 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other:

Sublethal observations / clinical signs:

Table 1: Mortality

Nominal concentration (mg/l)	Number of fish		Mortality (cumulated) after
			3h		6h		24h		48h		72h		96h
			V1	V2	V1	V2	V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	-	-	-	-	-	-	-	-	-	-	-	-
6.25	4	4	-	-	-	-	-	-	-	-	-	-	-	-
12.5	4	4	-	-	-	-	-	-	-	-	-	-	-	-
25	4	4	-	-	-	-	-	-	-	-	-	-	-	-
50	4	4	-	-	-	-	-	-	-	-	-	-	-	-
100	4	4	-	-	-	-	-	-	-	-	-	-	-	-

Table 2:Symptoms

Nominal concentration

(mg/l)

Number of fish

Symptoms (cumulated) after

24h

48h

72h

96h

Control

NS4

6.25

NS4

12.5

NS4

100

NS4

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

Table 3: pH

Nominal concentration (mg/l)	Number of fish		pH readings after
			24h		48h		72h		96h
			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	7.7	7.7	7.8	7.8	7.9	7.9	8.0	8.0
6.25	4	4	7.8	7.8	7.9	7.9	8.1	8.1	8.2	8.2
12.5	4	4	7.9	7.9	8.0	8.0	8.1	8.1	8.2	8.2
25	4	4	7.9	7.9	8.0	8.0	8.1	8.1	8.3	8.3
50	4	4	7.9	7.9	8.0	8.0	8.2	8.2	8.3	8.3
100	4	4	8.0	8.0	8.1	8.1	8.2	8.2	8.3	8.3

Table 4: oxygen content

Nominal concentration (mg/l)	Number of fish		Oxygen content (mg/l) after
			24h		48h		72h		96h
			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	7.0	7.0	6.9	6.9	6.8	6.8	6.7	6.7
6.25	4	4	6.8	6.8	6.7	6.7	6.6	6.6	6.5	6.5
12.5	4	4	6.8	6.8	6.7	6.7	6.6	6.6	6.5	6.5
25	4	4	6.8	6.8	6.7	6.7	6.6	6.6	6.5	6.5
50	4	4	6.8	6.8	6.7	6.7	6.6	6.6	6.5	6.5
100	4	4	6.8	6.8	6.7	6.7	6.6	6.6	6.5	6.5

Table 5: Test temperature

Nominal concentration (mg/l)	Number of fish		Temprature (°C) after
			24h		48h		72h		96h
			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	23.1	23.1	23.2	23.2	23.0	23.0	22.9	22.9
6.25	4	4	23.1	23.1	23.2	23.2	23.0	23.0	22.9	22.9
12.5	4	4	23.1	23.1	23.2	23.2	23.0	23.0	22.9	22.9
25	4	4	23.1	23.1	23.2	23.2	23.0	23.0	22.9	22.9
50	4	4	23.1	23.1	23.2	23.2	23.0	23.0	22.9	22.9
100	4	4	23.1	23.1	23.2	23.2	23.0	23.0	22.9	22.9

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No.56-34-8 on Zebra Fish Danio rerio was determine to be >100 mg/l.

Executive summary:

Study was conducted to assess the effect of test chemical Tetraethylammonium chloride (CAS No. 56-34-8) on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous stirring. This test solution was then added to the remaining three liters of water for achieving test concentrations of 6.25, 12.5, 25, 50 and 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was not hazardous and can be consider to be not classified as per the CLP classification criteria.

Description of key information

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No.56-34-8 on Zebra Fish Danio rerio was determine to be >100 mg/l.

Key value for chemical safety assessment

Fresh water fish

Effect concentration:: 100 mg/L

Additional information

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